期刊
MICROCHIMICA ACTA
卷 190, 期 10, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-023-05978-7
关键词
Porphyrins-based COF; Electrochemical detection; Modified electrode; Aptasensors; Electrochemical impedance spectroscopy; Escherichia coli
In this study, a two-dimensional porphyrin-based covalent organic framework was used to construct an aptamer-based electrochemical sensor for the detection of E.coli. The sensor demonstrated a low detection limit, excellent stability, and reproducibility, making it a promising technology for food safety applications.
The two-dimensional porphyrin-based covalent organic framework (denoted by Tph-TDC-COF) was used as the sensitive layerto build an aptamer-based electrochemical sensor for the detection of Escherichia coli (E.coli). Tph-TDC-COF produced with 5,10,15,20-tetrakis(4-aminophenyl)-21H, 23H-porphine (Tph) and [2,2 '-bithiophene]-2,5 '-dicarbaldehyde (TDC) as building blocks exhibited a highly conjugated structure, outstanding conductivity, large specific surface area, and strong bioaffinity towards aptamers. The adoption of Tph-TDC-COF-modified electrode resulted in improved sensing performance and increased anchoring affinity toward the E.coli-targeted aptamer. Under optimal conditions, the Tph-TDC-COF-based electrochemical aptasensor demonstrated an extremely low detection limit of 0.17 CFU mL(-1) for E.coli detection within a linear range of 10 to 1 x 10(8) CFU mL(-1), accompanied by good stability, excellent reproducibility and regeneration ability, and wide practical applications. The current electrochemical aptasensing technique has the potential to be extended to detect different foodborne bacteria using specific aptamer, therefore widening the application of COFs in biosensing and food safety fields.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据