期刊
MICROCHIMICA ACTA
卷 190, 期 8, 页码 -出版社
SPRINGER WIEN
DOI: 10.1007/s00604-023-05900-1
关键词
Peroxidase-like activity; Nanozyme; Glucose biosensor; Agarose hydrogel; Smartphone-based platform; Colorimetry
A novel magnetic nanozyme Fe3O4@ MXene-Au nanocomposite with higher peroxidase-like activity was developed. It exhibited outstanding magnetic properties, allowing for easy separation and great recyclability. A highly selective colorimetric biosensor for glucose detection was created using Fe3O4@ MXene-Au nanocomposites and glucose oxidase (Glu Ox).
A novel magnetic nanozyme Fe3O4@ MXene-Au nanocomposite, which possessed higher peroxidase-like activity than that of Fe3O4 nanoparticles and Fe3O4@ MXene nanocomposites, was developed. The outstanding magnetic properties of the nanozyme endowed it with the ability of simple and rapid separation, achieving great recyclability. Based on Fe3O4@ MXene-Au nanocomposites and glucose oxidase (Glu Ox), a highly selective colorimetric biosensor for glucose detection was developed. Fe3O4@ MXene-Au nanocomposites can catalyze H2O2 produced from glucose catalyzed by glucose oxidase to center dot OH and oxidize colorless 3,3',5,5'-tetramethylbenzidine (TMB) to blue oxidized TMB (oxTMB) with a significant absorbance at 652 nm. The linear range of glucose was 0-1.4 mM under optimal conditions, with a limit of detection (LOD) of 0.11 mM. Glucose in human whole blood was successfully detected with satisfactory recoveries. Furthermore, a facile agarose hydrogel detection platform was designed. With smartphone software, glucose detection can be realized by the agarose hydrogel platform, demonstrating the potential in on-site and visual detection of glucose.
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