期刊
MICROCHEMICAL JOURNAL
卷 193, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.microc.2023.109057
关键词
Bacteria detection; Molecular imprinting; Gold nanoparticle; Nanofilm; Optic sensor; Surface plasmon resonance
This study develops a biomimetic optic sensor for detecting Enterococcus faecalis in drinking water. The sensor utilizes a molecularly imprinted nanofilm made of gold nanoparticles. Extensive characterization shows that the sensor has remarkable detection capabilities with a low detection limit of 6.6x103 cfu/mL and a high determination coefficient within the Enterococcus faecalis concentration range of 5x104 - 1x108 cfu/mL. Importantly, the sensor is effective not only in buffer solutions but also in water sample solutions, making it practical for real-world applications.
This study presents the development of a biomimetic optic sensor for the detection of Enterococcus faecalis, a common fecal contaminant found in drinking water. The sensor utilizes a molecularly imprinted nanofilm, created by combining gold nanoparticles. Extensive characterization and analysis were conducted to evaluate the performance of the optic sensor, including kinetic, selectivity, reusability and real sample studies for real-time detection of Enterococcus faecalis. The results demonstrate that the optic sensor exhibits remarkable detection capabilities, offering a low detection limit of 6.6x103 cfu/mL and a high determination coefficient (R2 = 0.9886) within the Enterococcus faecalis concentration range of 5x104 - 1x108 cfu/mL. Importantly, the sensor proves effective not only in buffer solutions but also in water sample solutions, showcasing its practicality for real-world applications. Based on these findings, it can be concluded that the developed optic sensor shows promising potential for the detection of other bacteria, offering a cost-effective solution with enhanced sensitivity. Its versatility extends across diverse fields, including environmental monitoring and medical diagnosis. With its impressive performance, the optic sensor represents a valuable tool for ensuring water safety and advancing bacterial detection technologies.
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