A phenyl-functionalized nitrogen-rich magnetic hyper crosslinked polymer for magnetic solid-phase extraction of 5-nitroimidazoles residues in water and fish samples

A novel magnetic hyper crosslinked polymer (BTA-MHCP) was prepared by one-pot reaction of benzotriazole, 1,3,5-triphenylbenzene and phenyl-functionalized Fe3O4@SiO2. The BTA-MHCP was then investigated as the adsorbent for the rapid magnetic solid-phase extraction (MSPE) of the 5-nitroimidazoles (5-NDZs) including dimetridazole, metronidazole, secnidazole, ronidazole and ornidazole. The adsorbent showed good adsorption properties for the 5-NDZs. The key factors that affect the MSPE such as extraction time, the amount of sorbent, and the type of eluent and its volume were optimized. Under the optimal conditions, the linear response ranges for the established method were 0.15-100.0 ng mL-1 and 3.9-100.0 ng g-1 for drinking water and fish samples, respectively, with the coefficients of determination (r2) of 0.9981 to 0.9998. The limits of detection (S/N = 3) for the analytes were 0.02-0.05 ng mL-1 and 0.7-1.3 ng g-1, and the method recoveries ranged from 92.0% to 101.0% and from 89.9% to 104.0% for drinking water and fish samples, respectively.

Automated summary

A novel magnetic hyper crosslinked polymer (BTA-MHCP) was synthesized and used as an adsorbent for the rapid magnetic solid-phase extraction (MSPE) of 5-nitroimidazoles (5-NDZs). The key factors affecting the MSPE were optimized, and under the optimal conditions, the established method showed good linear response ranges, low limits of detection, and high method recoveries for both drinking water and fish samples.