Ancylobacter crimeensis sp. nov., a New Species of Aerobic Methylotrophic Bacteria Isolated from Oak Phyllosphere

A new facultative methylotroph, strain 6x-1(T), was isolated from the phyllosphere of oak (Quercus pubescens Willd.) on the medium with methanol. Cells were aerobic, gram-negative, not-spore-forming, non-motile short ovoids rods reproducing by binary fission. Growth optimum was at 25?29 degrees C and pH 7.0?7.5; growth was inhibited by 1.5% NaCl. Predominant fatty acids were C-18:1?7c and C(19:0)cyclo. Predominant phospholipids were phosphatidylethanolamine, phosphatidylmonomethylethanolamine, and diphosphatidylglycerol. The major ubiquinone was Q(10). The 16S rRNA gene sequence of strain 6x-1T exhibited the highest similarity to those of members of the genus Ancylobacter (97.0?97.4%). Genome analysis of strain 6x-1(T) and most closely related Ancylobacter strains revealed that the values of ANI (80.7?83.5%), dDDH (22.4?23.3%), AAI (72.0?78.0%), and POCP (62.0?69.0%) were below the recommended threshold values for prokaryotic species. Genome size of strain 6x-1(T) was 4.29 Mb, and G + C content was 67.3%. According to the results of phylogenetic, phylogenomic, phenotypic, and chemotaxonomic analysis, strain 6x-1T (=(VKM ?-3256(T) = CCUG 72401(T) = ?C?C 92567(T))) represents a new species of genus Ancylobacter, for which the name Ancylobacter crimeensis sp. nov. is proposed.

Automated summary

A new facultative methylotroph strain, 6x-1(T), was isolated from the phyllosphere of oak using methanol as a medium. It belongs to gram-negative short ovoid rods and has specific physiological and chemical characteristics. Based on gene sequence and genome analysis, this strain represents a new species of the genus Ancylobacter.