4.5 Article

Effective of phage cocktail against Klebsiella pneumoniae infection of murine mammary glands

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MICROBIAL PATHOGENESIS
卷 182, 期 -, 页码 -

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ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2023.106218

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Mastitis; Phage cocktails; Klebsiella pneumoniae; Mice

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This study aimed to create a phage cocktail using 3 Klebsiella lytic phages and compare its bactericidal activity against antibiotic-resistant bacteria causing bovine mastitis with an individual phage. The results showed that the phage cocktail had better efficiency in both in vitro and in vivo experiments.
Phage therapy has potential to combat antibiotic-resistant bacteria causing bovine mastitis. Our objective was to use 3 Klebsiella lytic phages to create a phage cocktail, and to compare bactericidal activity of this phage cocktail versus an individual phage, both in vitro and in vivo. Based on transmission electron microscopy, phage CM_Kpn_HB154724 belonged to Podoviridae and on double agar plates, it formed translucent plaques on the bacterial lawn of Klebsiella pneumoniae KPHB154724. In one-step growth curves, this phage had a latent period of 40 min, an outbreak period of 40 min, a burst size of 1.2 x 107 PFU/mL, and an optimal multiplicity of infection (MOI) of 1. Furthermore, it was inactivated under extreme conditions (pH & LE; 3.0 or & GE; 12.0 and temperatures of 60 or 70 degrees C). It had a host range of 90% and had 146 predicted genes (Illumine NovaSeq). Based on histopa-thology and expression of inflammatory factors interleukin-1 & beta;, tumor necrosis factor-& alpha;, interleukin-6, and prostaglandin, phage cocktail therapy had better efficiency than an individual phage in K. pneumoniae-infected murine mammary glands. In conclusion, we used 3 Klebsiella lytic phages to create a phage cocktail and confirmed its effectiveness against K. pneumoniae both in vitro (bacterial lawn) and in vivo (infected murine mammary glands).

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