Pathogenicity characterization of PRRSV-1 181187-2 isolated in China

PRRSV-1 has caused more clinical infections in pigs in Chinese swine herds in recent years, however, the pathogenicity of PRRSV-1 in China is unclear. In order to study the pathogenicity of PRRSV-1, in this study, a PRRSV-1 strain, 181187-2, was isolated in primary alveolar macrophage (PAM) cells from a farm where abor-tions had been reported in China. The complete genome of 181187-2 was 14932 bp excluding Poly A, with 54 -amino acid continuous deletion in the Nsp2 gene and 1 amino deletion in ORF3 gene compared with LV. Additionally, the piglets inoculated with strain 181187-2 by intranasal and intranasal plus intramuscular in-jection, animal experiments showed clinical symptoms including transient fever and depression, with no death. The obvious histopathological lesions including interstitial pneumonia and lymph node hemorrhage, and there were no significant differences in clinical symptoms and histopathological lesions with different challenge ways. Our results indicated that PRRSV-1 181187-2 was a moderately pathogenic strain in piglets.

Automated summary

PRRSV-1 has caused more clinical infections in Chinese swine herds and its pathogenicity in China remains unclear. In this study, a PRRSV-1 strain, 181187-2, was isolated from a farm with abortion reports in China. The results showed that PRRSV-1 181187-2 exhibited moderate pathogenicity in piglets.