Effect of resveratrol on skeletal slow-twitch muscle fiber expression via AMPK/PGC-1 & alpha; signaling pathway in bovine myotubes

The purpose of this study was to evaluate the impact of resveratrol on slow-twitch muscle fiber expression in bovine myotubes. The results revealed that resveratrol enhanced slow myosin heavy chain (MyHC) and suppressed fast MyHC protein expression, accompanied by increased MyHC I/IIa and decreased MyHC IIx/IIb mRNA levels in bovine myotubes (P < 0.05). Resveratrol also enhanced the activities of succinic dehydrogenase (SDH), malate dehydrogenase (MDH) and the mitochondrial DNA (mtDNA) content, but reduced lactate dehydrogenase (LDH) activity (P < 0.05). Meanwhile, the protein and gene expression of AMPK, SIRT1 and PGC-1a were upregulated by resveratrol (P < 0.05). Furthermore, PGC-1a inhibitor SR-18292 could attenuate resveratrolinduced muscle fiber conversion from fast-twitch to slow-twitch. These results suggest that resveratrol might promote muscle fiber type transition from fast-twitch to slow-twitch through the AMPK/PGC-1a signaling pathway and mitochondrial biogenesis in bovine myotubes.

Automated summary

The purpose of this study was to evaluate the impact of resveratrol on slow-twitch muscle fiber expression in bovine myotubes. The results showed that resveratrol enhanced the expression of slow myosin heavy chain (MyHC) and suppressed the expression of fast MyHC in bovine myotubes. Additionally, resveratrol increased the activities of succinic dehydrogenase (SDH) and malate dehydrogenase (MDH), as well as the mitochondrial DNA (mtDNA) content, while reducing lactate dehydrogenase (LDH) activity. Moreover, resveratrol upregulated the expression of AMPK, SIRT1, and PGC-1a. These findings suggest that resveratrol promotes the transition of muscle fiber type from fast-twitch to slow-twitch in bovine myotubes through the AMPK/PGC-1a signaling pathway and mitochondrial biogenesis.