Comparative Transcriptome and WGCNA Analysis Reveal Molecular Responses to Salinity Change in Larvae of the Iwagaki Oyster Crassostrea Nippona

The Iwagaki oyster Crassostrea nippona is an important aquaculture species with significant potential for large-scale oyster farming. It is susceptible to the fluctuated salinity in the coastal area. In this study, we compared the transcriptome of Crassostrea nippona larvae under variant conditions with low-salinity stress (28, 20, 15, 10, and 5 practical salinity units (psu)) for 24 h. KEGG enrichment analysis of differentially expressed genes (DEGs) from pairwise comparisons identified several free amino acid metabolism pathway (taurine and hypotaurine, arginine and proline, glycine, and beta-alanine) contributing to the salinity change adaptation and activated lysosome and apoptosis pathway in response to the low-salinity stress (10 and 5 psu). Trend analysis revealed sustained upregulation of transmembrane transport-related genes (such as SLC family) and downregulation of ribosomal protein synthesis genes faced with decreasing salinities. In addition, 9 biomarkers in response to low-salinity stress were identified through weighted gene co-expression network analysis (WGCNA) and validated by qRT-PCR. Our transcriptome analysis provides a comprehensive view of the molecular mechanisms and regulatory networks underlying the adaptive responses of oyster larvae to hypo-salinity conditions. These findings contribute to our understanding of the complex biological processes involved in oyster resilience and adaptation to changing environmental conditions.

Automated summary

This study investigated the adaptive responses of Crassostrea nippona larvae to low-salinity conditions using transcriptome analysis. The results revealed that the oyster larvae adapted to low-salinity stress by regulating free amino acid metabolism pathways and activating lysosome and apoptosis pathways. In addition, transmembrane transport-related genes were upregulated, while ribosomal protein synthesis genes were downregulated in response to decreasing salinities. Nine biomarkers in response to low-salinity stress were identified and validated. The findings provide valuable insights into the molecular mechanisms underlying the resilience and adaptation of oyster larvae to changing environmental conditions.