期刊
MOLECULAR CELL
卷 61, 期 6, 页码 874-885出版社
CELL PRESS
DOI: 10.1016/j.molcel.2016.02.027
关键词
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资金
- Ministry of Education, Culture, Sports, Science, and Technology [00557069, 24115711, 26113007]
- Nakajima Foundation
- Mochida Memorial Foundation for Medical and Pharmaceutical Research
- Daiichi-Sankyo Foundation of Life Science
- Grants-in-Aid for Scientific Research [24115711, 26113007, 15K18476] Funding Source: KAKEN
The control of mRNA stability plays a central role in regulating gene expression. In metazoans, the earliest stages of development are driven by maternally supplied mRNAs. The degradation of these maternal mRNAs is critical for promoting the maternal-to-zygotic transition of developmental programs, although the underlying mechanisms are poorly understood in vertebrates. Here, we characterized maternal mRNA degradation pathways in zebrafish using a transcriptome analysis and systematic reporter assays. Our data demonstrate that ORFs enriched with uncommon codons promote deadenylation by the CCR4-NOT complex in a translationdependent manner. This codon-mediated mRNA decay is conditional on the context of the 30 UTR, with long 30 UTRs conferring resistance to deadenylation. These results indicate that the combined effect of codon usage and 30 UTR length determines the stability of maternal mRNAs in zebrafish embryos. Our study thus highlights the codon-mediated mRNA decay as a conserved regulatory mechanism in eukaryotes.
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