期刊
MOLECULAR CELL
卷 61, 期 2, 页码 260-273出版社
CELL PRESS
DOI: 10.1016/j.molcel.2015.12.001
关键词
-
资金
- EMBL Interdisciplinary Postdoc (EIPOD) fellowship under Marie Curie Actions (COFUND)
- UK Biotechnology and Biological Sciences Research Council (BBSRC)
- European Commission's FP7 project RADIANT
- Max Planck Society
- German Research Foundation (DFG) concerted research consortium CRC992 Medical Epigenetics''
- Federal Ministry of Education and Research (BMBF) under the DEEP consortium
- [PO1 GM085354]
- Biotechnology and Biological Sciences Research Council [1112564] Funding Source: researchfish
DNA replication is temporally and spatially organized in all eukaryotes, yet the molecular control and biological function of the replication-timing program are unclear. Rif1 is required for normal genome-wide regulation of replication timing, but its molecular function is poorly understood. Here we show that in mouse embryonic stem cells, Rif1 coats late-replicating domains and, with Lamin B1, identifies most of the late-replicating genome. Rif1 is an essential determinant of replication timing of non-Lamin B1-bound late domains. We further demonstrate that Rif1 defines and restricts the interactions between replication-timing domains during the G1 phase, thereby revealing a function of Rif1 as organizer of nuclear architecture. Rif1 loss affects both number and replication-timing specificity of the interactions between replication-timing domains. In addition, during the S phase, Rif1 ensures that replication of interacting domains is temporally coordinated. In summary, our study identifies Rif1 as the molecular link between nuclear architecture and replication-timing establishment in mammals.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据