Triploid burbot, Lota lota, production: Optimization of thermal and hydrostatic parameters, tetraploid induction, and confirmation of triploid sterility

Experiments were conducted to optimize triploid induction parameters, and assess triploid sterility, in burbot. Duration and timing of shocks were based on degree minutes, temperature multiplied by time, denoted as degrees C min. Hydrostatic shock experiments investigated the duration of shock using 7500 or 8500 psi at 180 degrees C min postfertilization. Thermal shocks investigated duration of shock and post-fertilization shock timing using a shock of 16 degrees C. Sterility experiments investigated egg survival when diploids were crossed with triploids. Hydrostatic shock of 7500 psi for 10 or 20 degrees C min can induce triploidy >= 90% and exhibits survival that is statistically similar, p <= 0.05, to controls. Hydrostatic shock of 8500 psi for 5 or 10 degrees C min yielded triploid induction of 93% and 100%, respectively, with survival that is statistically similar to controls, p <= 0.05. Thermal induction experiments indicated shocks at 120 degrees C min post-fertilization, for durations between 350 and 450 degrees C min, have potential to induce triploidy >= 90% while facilitating survival statistically similar to controls, p <= 0.05. Induction of tetraploidy was observed. Sterility experiments determined that triploid burbot are functionally sterile. These results may allow production of burbot where sterility is required.

Automated summary

Experiments were conducted to optimize triploid induction parameters in burbot, and it was found that hydrostatic shocks and thermal shocks could effectively induce triploidy with high percentages. The survival rates of triploid burbot were statistically similar to the control group. The sterility experiments confirmed that triploid burbot are functionally sterile. These results are significant for the production of sterile burbot.