Materialization of a Novel Decorated Nanowire Biosensor Platform Based on Field Effect Transistor under Electrochemical Gate Modulation

The highly sensitive and cost-free field effect transistor (FET) biosensor for specific detection of DNA and streptavidin based on Zinc Oxide Nanowires (NWs) to develop the next generation biosensors in label-free approach under electrochemical gate modulation has been materialized. The electronic properties of NWs were changed by binding of charged analytes to NWs surface under electrochemical gate modulation. Due to highly chemically inertness, immobilization of the target analyte receptors on the NWs could be conducted through electrochemical treatment. For materialization of DNA binding, gold nanoparticles (Au NPs) were used to functionalize ZnO NWs surface. In this work, a liquid gated FET based on NWs was configured to detect the complementary DNA strand hybridization as well as the protein streptavidin at very low concentration. The biosensor could reach to sensitivity of attomolar. The sensitivity of response was check and biosensor showed the sensitivity ranges from 10(-18) to 10(-12) mol.L-1. The FET biosensor could distinguish a complementary strand from a single nucleotide polymorphism (SNP) containing strand. The limit of detection (LOD) reached to15 aM. The NPs decoration on the NWs surface brought about the high detection limit. The NPs decoration was being a promising method and scientific approach for biomolecules detection.

Automated summary

A highly sensitive and cost-free field effect transistor (FET) biosensor based on zinc oxide nanowires (NWs) has been developed for the specific detection of DNA and streptavidin. Under electrochemical gate modulation, the electronic properties of the NWs were altered by the binding of analytes, allowing for the immobilization of target analyte receptors through electrochemical treatment. The biosensor demonstrated attomolar sensitivity and could differentiate between a complementary DNA strand and a single nucleotide polymorphism (SNP).