期刊
MOLECULAR BIOLOGY OF THE CELL
卷 27, 期 21, 页码 3357-3368出版社
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E16-07-0506
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资金
- National University of Singapore [R-154-000-515-133, R-154-000-524-651, D-E12-303-154-217, YIA R-154-000-558-133, MOE T2 R-154-000-624-112]
- Biomedical Research Council of AstarSTAR (Agency of Science Technology and Research), Singapore
- Japan Science and Technology Agency CREST grant
- Ministry of Education, Culture, Sports, Science and Technology KAKENHI grant [23115005]
- Grants-in-Aid for Scientific Research [16H04746] Funding Source: KAKEN
Chromatin organization has an important role in the regulation of eukaryotic systems. Although recent studies have refined the three-dimensional models of chromatin organization with high resolution at the genome sequence level, little is known about how the most fundamental units of chromatin-nucleosomes-are positioned in three dimensions in vivo. Here we use electron cryotomography to study chromatin organization in the budding yeast Saccharomyces cerevisiae. Direct visualization of yeast nuclear densities shows no evidence of 30-nm fibers. Aside from preribosomes and spindle microtubules, few nuclear structures are larger than a tetranucleosome. Yeast chromatin does not form compact structures in interphase or mitosis and is consistent with being in an open configuration that is conducive to high levels of transcription. From our study and those of others, we propose that yeast can regulate its transcription using local nucleosome-nucleosome associations.
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