期刊
JOURNAL OF SEPARATION SCIENCE
卷 46, 期 22, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jssc.202300196
关键词
differential metabolites; Ligustri Lucidi Fructus; multivariate statistical analysis
By conducting a metabolomics analysis, the optimal harvest period of Ligustri Lucidi Fructus (LLF) was determined and indicators for evaluating its maturity were identified, providing important guidance for the medicinal market.
After medicinal market research, it was found that the harvest time of Ligustri Lucidi Fructus (LLF) was chaotic in practice. In order to determine the optimal harvest period of LLF to ensure its pharmacological activity, metabolomics analysis of LLF at different harvest times based on ultra-high-performance liquid chromatography-triple quadrupole-(linear ion trap)-tandem mass spectrometry was established. In this study, 166 differential metabolites (DMs) in 448 metabolites at different harvest times were screened out based on variable importance in projection value, and among them, 94 DMs with regular trends of change in relative content (59 increased and 35 decreased with the growth period) were chosen to further research. The result of the multivariate statistical analysis showed that November was the optimal harvest period of LLF. Additionally, 10-hydroxyligustroside, oleoside 11-methyl ester, and salidroside were screened out to be used as the evaluation indicators of immature LLF, while specnuezhenide, nuezhenoside G13, and neonuezhenide were the evaluation indicators of mature LLF. This study provides fundamental insight for metabolite identification and proposes the best harvest period of LLF to avoid confusion in the medicinal market.
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