☆ 4.5 Review

Open tubular liquid chromatography: Recent advances and future trends

JOURNAL OF SEPARATION SCIENCE (2023)

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Practical aspects of preparation, optimization, and evaluation of poly (styrene-divinylbenzene)porous-layer open tubular (PLOT) columns for capillary liquid chromatography

Juliana Soares da Silva Burato et al.

Summary: Using open tubular (OT) capillary columns, specifically the PLOT (porous layer open tubular) type, offers advantages in terms of interaction with analytes in liquid chromatography. This study explores the application of PLOT columns in capillary LC and discusses the preparation, optimization, and evaluation of these columns. Poly(styrene-divinylbenzene) (PS-DVB) was used as the stationary phase, and comparisons were made between columns with internal diameters of 10 and 25 μm. The effects of flow rate and gradient window were also investigated.

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Article Chemistry, Analytical

Novel open-tubular columns with highly hydrophobic stationary phase for peptide and protein separation by nano-liquid chromatography

Buesra Beltekin et al.

Summary: In this study, novel open-tubular nano-columns with highly hydrophobic stationary phase were prepared via surface graft polymerization. The columns showed satisfactory separation of alkylbenzenes and high-resolution analysis of intact proteins and peptides.

SEPARATION SCIENCE PLUS (2023)

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Article Chemistry, Analytical

Compact capillary high performance liquid chromatography system for pharmaceutical on-line reaction monitoring

Lewellwyn J. Coates et al.

Summary: Due to their size, conventional high performance liquid chromatographs (HPLCs) are difficult to place close to a reaction vessel within a pharmaceutical manufacturing or development site. However, a compact and modular separation system using capillary HPLC has been developed to enable co-location of an HPLC with a small-scale reactor for reaction monitoring. The compact system showed equivalent quantitative performance to an Agilent 1290 Infinity II HPLC system.

ANALYTICA CHIMICA ACTA (2023)

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Preparation and evaluation of ultra-long open-tubular capillary columns modified with zeolitic imidazolate framework-8 incorporated polymeric porous layer for liquid chromatography

Zhihua Zhong et al.

Summary: An ultra-long open tubular capillary liquid chromatographic column incorporating ZIF-8 was prepared and evaluated. The column showed high column efficiency and improved chromatography performance with the incorporation of ZIF-8. The separation was achieved through multiple interactions and exhibited excellent repeatability and potential applications.

JOURNAL OF CHROMATOGRAPHY A (2022)

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Development of Ultranarrow-Bore Open Tubular High Efficiency Liquid Chromatography

Yingyan Zhou et al.

Summary: Chromatography theory suggests that ultranarrow-bore open tubular liquid chromatography holds advantages in small sample requirement and high separation efficiency, but also presents challenges. Research efforts aim to mature its application in ultra-small sample analysis like single-cell and subcellular organelles omics research.

CHINESE JOURNAL OF CHEMISTRY (2022)

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Electron ionization mass spectrometry: Quo vadis?

Edvaldo Vasconcelos Soares Maciel et al.

Summary: Mass spectrometry (MS) is a fundamental technique for compound identification based on mass-to-charge ratio. Electron ionization (EI) is a widely used ionization technique that can generate unique spectra for each molecule. It is often combined with chromatography and researchers are working on developing interfaces for coupling liquid chromatography (LC) and EI-MS.

ELECTROPHORESIS (2022)

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Article Biochemical Research Methods

Open tubular nano-liquid chromatography with a new polylysine grafted on graphene oxide stationary phase for the separation and determination of casein protein variants in milk

Sibel Seker et al.

Summary: A novel narrow capillary coating procedure was reported for the preparation and application of open-tubular (OT) column for the separation of casein protein variants in milk. A new poly-L-lysine grafted on graphene oxide stationary phase was developed for OT nano-liquid chromatography. The developed OT column showed promising performance in the separation of casein protein variants.

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Porous layer open tubular nano liquid chromatography directly coupled to electron ionization mass spectrometry

Deyber Arley Vargas Medina et al.

Summary: A 25 μm i.d x 1.2 m length PS-DVB porous layer open tubular column (PLOT) was prepared and assessed in the configuration of a nano liquid chromatography coupled to an electron ionization mass spectrometry system (OT-nanoLC-EI-Ms). The system demonstrated competitive applicability in separating non-amenable ESI compounds, such as polyaromatic hydrocarbons (PAHs) and non-amenable GC compounds such as thermolabile pesticides. With excellent chromatographic performance, the PLOT columns can work under more compatible EI-detection conditions.

JOURNAL OF CHROMATOGRAPHY A (2022)

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Article Biochemical Research Methods

Open tubular liquid chromatographic system for using columns with inner diameter of 2 mu m. A tutorial

Apeng Chen et al.

Summary: This study demonstrates the excellent performance of liquid chromatography using 2-mu m-i.d. open tubular columns, with peak capacities of over 2000 achieved in less than three hours. The tutorial provides guidance on setting up an open tubular liquid chromatography system, including key components, construction, advantages, disadvantages, and practical issues related to using narrow columns.

JOURNAL OF CHROMATOGRAPHY A (2022)

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Review Chemistry, Analytical

Novel Pumping Methods for Microfluidic Devices: A Comprehensive Review

Aleksei P. Iakovlev et al.

Summary: This review discusses methods for controlling microfluidic flow with high accuracy using various strategies. The systems are divided into two groups - passive and active - based on their flow creation mechanisms. The operation principles, advantages, and disadvantages of each group are explained. The focus is on mechanical systems, which are of particular interest due to their precision flow control and autonomous operation. The integration of autonomous microfluidic systems with smartphones or single-board computers, as well as future trends and potential developments in the field, are also discussed.

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Article Biochemical Research Methods

Preparation and Evaluation of 2 m Long Open Tubular Capillary Columns of 50 μm Internal Diameter for Separation of Peptides in Liquid Chromatography

Ashraf Ali et al.

Summary: An open tubular column was prepared with co-polymer layer on the inner surface of a 2m long silica capillary, exhibiting high separation efficiency for peptides. By modifying the capillary surface, introducing initiator, and conducting reversible polymerization, the column produced a high number of theoretical plates and showed good repeatability.

CHROMATOGRAPHIA (2021)

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Article Biochemical Research Methods

A stationary pseudophase semi-permanent coating for open-tubular capillary liquid chromatography and electrochromatography

Mohamed Adel Ahmed et al.

Summary: This study described the chromatographic and electrochromatographic separation of small neutral and charged analytes using a fused silica capillary with a stationary pseudophase semi-permanent coating of DDAB aggregates. The research showed the formation of DDAB patchy admicelle, complete admicelle, or larger aggregates inside the capillary, depending on the concentration of DDAB used in coating. Overall, the use of a stationary pseudophase coating provides a potentially easier alternative way to conduct open-tubular liquid chromatography and electrochromatography.

JOURNAL OF CHROMATOGRAPHY A (2021)

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Small footprint liquid chromatography-mass spectrometry for pharmaceutical reaction monitoring and automated process analysis

Mohamed Hemida et al.

Summary: A portable capillary liquid chromatography (LC) system has been developed for on-site pharmaceutical analysis, providing low detection limits and a wide dynamic range. This system demonstrated robust performance for automated process analysis, improving throughput and understanding of synthetic processes.

JOURNAL OF CHROMATOGRAPHY A (2021)

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Article Chemistry, Analytical

Liquid Chromatographic Separation Using a 2 μm i.d. Open Tubular Column at Elevated Temperature

Yu Yang et al.

Summary: Increasing the operating temperature of the liquid chromatography column can significantly enhance its efficiency, peak capacity, and separation speed without compromising one over the other. Experimental findings using a mixture of amino acids show impressive improvements in efficiency and speed when the temperature is raised.

ANALYTICAL CHEMISTRY (2021)

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The influence of injection volume on efficiency of microbore liquid chromatography columns for gradient and isocratic elution

Tobias Werres et al.

Summary: Injection volume and column volume overload are common issues in miniaturized chromatography. Optimal injection volume balancing chromatographic efficiency and sensitivity is crucial. Experimental results show that volume overload in isocratic elution can decrease chromatographic efficiency, while a reduction in peak capacity in gradient elution is only observed when the injection volume exceeds 40% of the effective column volume.

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Article Biochemical Research Methods

An overview of open tubular liquid chromatography with a focus on the coupling with mass spectrometry for the analysis of small molecules

Deyber Arley Vargas Medina et al.

Summary: Open tubular liquid chromatography (OT-LC) offers superior chromatographic performance and more favorable mass spectrometry detection conditions, particularly when coupled with electrospray ionization sources (ESI). OT-LC-MS is mainly utilized in proteomics and biosciences analysis, but can also provide enhanced sensitivity and superior chromatographic performance in analyzing small molecules.

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Online In-Tube Solid-Phase Microextraction Coupled to Liquid Chromatography-Tandem Mass Spectrometry for the Determination of Tobacco-Specific Nitrosamines in Hair Samples

Atsushi Ishizaki et al.

Summary: This study developed a method for simultaneous determination of five TSNAs in hair with high sensitivity and specificity, which can be used to assess long-term exposure levels to tobacco smoke in smokers and non-smokers. The method allows for easy measurement of TSNA levels using 5 mg hair samples.

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In-situ growth of a spherical vinyl-functionalized covalent organic framework as stationary phase for capillary electrochromatography-mass spectrometry analysis

Wenqi Sun et al.

Summary: The COF-V based CEC column demonstrates strong electroosmotic flow, high separation efficiency, and stability. It exhibits powerful separation selectivity to various compounds and excellent repeatability, making it suitable for complex sample analysis.

TALANTA (2021)

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Review Chemistry, Analytical

Liquid chromatography using ≤5 μm open tubular columns

Piliang Xiang et al.

Summary: Significant progresses have been made recently in liquid chromatography using <= 5-mu m-i.d. open tubular columns, enabling peak capacities of 2000+, efficiencies of 10+ million plates/m, and millisecond separations. This review article summarizes the latest advancements, procedures for preparing columns, selecting experimental components, and discusses the future prospects of this technique.

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Current prospects on nano liquid chromatography coupled to electron ionization mass spectrometry (nanoLC-EI-MS)

Deyber Arley Vargas Medina et al.

Summary: This article reviews the development of nanoLC technology and its integration with EI-MS, exploring new advances and discussing limitations in the field. It provides the reader with a current overview of nanoLC technology and highlights future research directions.

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Miniature and fully portable gradient capillary liquid chromatograph

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Performing flow injection chromatography using a narrow open tubular column

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Article Chemistry, Analytical

LED-Based UV Absorption Detector with Low Detection Limits for Capillary Liquid Chromatography

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ANALYTICAL CHEMISTRY (2015)

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Article Chemistry, Analytical

Integration of uniform porous shell layers in very long pillar array columns using electrochemical anodization for liquid chromatography

Manly Callewaert et al.

ANALYST (2014)

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Article Chemistry, Analytical

3D printed metal columns for capillary liquid chromatography

S. Sandron et al.

ANALYST (2014)

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Article Chemistry, Analytical

An Open Tubular Ion Chromatograph

Bingcheng Yang et al.

ANALYTICAL CHEMISTRY (2014)

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Article Biochemical Research Methods

Development of a C60-fullerene bonded open-tubular capillary using a photo/thermal active agent for liquid chromatographic separations by π-π interactions

Takuya Kubo et al.

JOURNAL OF CHROMATOGRAPHY A (2014)

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Article Biochemical Research Methods

Evaluation of ionic liquids-coated carbon nanotubes modified chiral separation system with chondroitin sulfate E as chiral selector in capillary electrophoresis

Qi Zhang et al.

JOURNAL OF CHROMATOGRAPHY A (2014)

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Article Biochemical Research Methods

3D printed porous media columns with fine control of column packing morphology

Conan Fee et al.

JOURNAL OF CHROMATOGRAPHY A (2014)

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Article Biochemical Research Methods

Instrumentation for hand-portable liquid chromatography

Sonika Sharma et al.

JOURNAL OF CHROMATOGRAPHY A (2014)

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Article Biochemical Research Methods

Performance evaluation of thick film open tubular silica capillary by reversed phase liquid chromatography

Simon Forster et al.

JOURNAL OF CHROMATOGRAPHY A (2013)

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Article Biochemical Research Methods

Preparation and kinetic performance assessment of thick film 10-20 μm open tubular silica capillaries in normal phase high pressure liquid chromatography

Simon Forster et al.

JOURNAL OF CHROMATOGRAPHY A (2013)

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Article Chemistry, Analytical

Separation optimization of long porous-layer open-tubular columns for nano-LC-MS of limited proteomic samples

Magnus Rogeberg et al.

JOURNAL OF SEPARATION SCIENCE (2013)

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Article Chemistry, Analytical

Capillary column coated with graphene oxide as stationary phase for gas chromatography

Qishu Qu et al.

ANALYTICA CHIMICA ACTA (2012)

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Article Biochemical Research Methods

Performance characteristics of new superficially porous particles

Joseph J. DeStefano et al.

JOURNAL OF CHROMATOGRAPHY A (2012)

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Article Chemistry, Analytical

Parallel, fluorous open-tubular chromatography using microstructured fibers

Adam B. Daley et al.

ANALYTICA CHIMICA ACTA (2011)

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Article Biochemical Research Methods

Portable, lightweight, low power, ion chromatographic system with open tubular capillary columns

Isaac K. Kiplagat et al.

JOURNAL OF CHROMATOGRAPHY A (2010)

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Article Chemistry, Analytical

Nanocapillaries for Open Tubular Chromatographic Separations of Proteins in Femtoliter to Picoliter Samples

Xiayan Wang et al.

ANALYTICAL CHEMISTRY (2009)

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Article Chemistry, Analytical

Bare nanocapillary for DNA separation and genotyping analysis in gel-free solutions without application of external electric field

Xiayan Wang et al.

ANALYTICAL CHEMISTRY (2008)

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Article Biochemical Research Methods

Chromatographic separations in a nanocapillary under pressure-driven conditions

Xiayan Wang et al.

JOURNAL OF CHROMATOGRAPHY A (2008)

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Article Chemistry, Analytical

Ultratrace LC/MS proteomic analysis using 10-μm-i.d.: Porous layer open tubular poly(styrene-divinylbenzene) capillary columns

Guihua Yue et al.

ANALYTICAL CHEMISTRY (2007)

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Review Chemistry, Analytical

A practical guide to nano-LC troubleshooting

Marek Noga et al.

JOURNAL OF SEPARATION SCIENCE (2007)

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Article Chemistry, Analytical

Domain size-induced heterogeneity as performance limitation of small-domain monolithic columns and other LC support types

Jeroen Billen et al.

ANALYTICAL CHEMISTRY (2006)

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Article Automation & Control Systems

The electrolysis of water: an actuation principle for MEMS with a big opportunity

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MECHATRONICS (2000)

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