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Engineering, Chemical
Daisy Yang et al.
Summary: This study successfully applied an open microfluidic chip for real-time continuous monitoring of EDTA extractable iron in mineral slurries, providing a convenient and efficient analysis method for depressing mineral flotation in mineral processing.
CANADIAN JOURNAL OF CHEMICAL ENGINEERING
(2023)
Article
Biochemical Research Methods
Tomas Vaclavek et al.
Summary: Analysis of cellular composition and metabolism at a single-cell resolution is important for understanding complex relationships within tissues or organisms. Mass spectrometry (MS) is an ideal tool for detecting and identifying compounds in low-volume samples with high chemical complexity. This study presents a microfluidic sampling platform that allows single-cell extraction from MS-incompatible media and analysis of human erythrocytes using electrical cell lysis and nanoESI-MS. Hemoglobin alpha and beta chains were successfully identified in single-erythrocyte lysates.
Article
Engineering, Chemical
Niamh A. Kilcawley et al.
Summary: A multi-step assay was automated using Lab-on-a-Disc (LoaD) technology, combining buoyancy pumping and dissolvable film. The technology allows tasks like blood centrifugation to be located in less valuable areas with higher centrifugal forces. A mathematical model and simulation were used to estimate volume errors caused by manufacturing deviations.
Article
Chemistry, Analytical
Le Tran Huy Thang et al.
Summary: In this study, a pressure-driven and self-contained cartridge with pre-stored reagents was designed to automate nucleic acid purification. By using pressurized air stored inside the cartridge, reagents were transferred through a microfluidic chip, which reduced operational complexity. The device achieved similar results to the conventional column-based extraction method in terms of concentration, purity, and real-time polymerase chain reaction (qPCR) for Escherichia coli (E. coli) O157:H7 DNA. It also had a detection limit of 103 CFU, similar to the conventional solid-phase extraction method. This novel approach for automating sample preparation can facilitate point-of-care molecular diagnostics.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Tianlong Zhang et al.
Summary: Here, we demonstrate the separation and enrichment of Escherichia coli clusters from its singlets using a viscoelastic microfluidic device. The ability to obtain highly pure populations of E. coli clusters is crucial for various applications in biology, biomedicine, and industry. Experimental results show that particles of different sizes migrate towards the center of the channel due to the combined effects of inertial and elastic forces. The size-dependent separation efficiency is influenced by channel length, sheath-to-sample flow rate ratio, and poly(ethylene oxide) concentration.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Zongliang Quan et al.
Summary: The constant mutation and emergence of new types of microorganisms pose significant challenges to their detection. This study achieved high-resolution whole protein 2D microfluidic chip electrophoresis and explored the feasibility of identifying and semiqualifying unknown microbes. The results demonstrate the promising prospects of microfluidic chip electropherogram fingerprint-based quick microorganism assays, biointeraction studies, and drug screenings.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Nanoscience & Nanotechnology
Jiachen Yang et al.
Summary: This paper presents a highly integrated immunoturbidimetric assay for immune globulin identification from whole blood sample. The system combines blood cell sedimentation and on-site extraction of purified plasma on a chip, resulting in a low-cost, portable, and time-saving platform.
MICROFLUIDICS AND NANOFLUIDICS
(2022)
Article
Chemistry, Multidisciplinary
Gaowa Xing et al.
Summary: This article discusses the advances in bacteria detection using microfluidic biosensors. It introduces typical methods for pathogenic bacteria capture, separation, and detection, as well as key applications of microfluidic biosensor-based rapid bacteria detection. Furthermore, it concludes and discusses possible research prospects in the field of microfluidic biosensors for the rapid detection of pathogenic bacteria.
CHINESE CHEMICAL LETTERS
(2022)
Article
Chemistry, Analytical
Antoine Enel et al.
Summary: This article presents a new DMFP platform for handling gaseous samples, involving a two-step digital preconcentration to increase preconcentration factors. By bypassing the breakthrough volume limit, the system enables easier monitoring of model compounds and promising applications in volatile compound analysis devices.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Zhenna Chen et al.
Summary: A negative magnetophoresis focusing microchip was developed and coupled to ICP-MS for single-cell analysis, achieving high sample throughput. Study on uptake behaviors revealed that MCF-7 cells exhibited more remarkable heterogeneity when treated with ZnO NPs, with lower uptake content compared to Zn2+.
ANALYTICAL CHEMISTRY
(2022)
Article
Biophysics
Yujin Xiao et al.
Summary: MDCM technology allows rapid and accurate dispensing of multiple reagents and samples, making it a high-potential platform for POCT.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Environmental Sciences
Hamsa N. Gowda et al.
Summary: The study developed a portable pathogen analysis system that can detect bacteria in water within 1 hour, with the advantages of simple operation and high sensitivity.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Chemistry, Analytical
Wen-Jing Hao et al.
Summary: In this study, a microfluidic platform-based enrichment strategy was proposed, combining molecularly imprinted polymer (MIPs) with post-imprinting modification and trypsin-immobilized enzyme reactor (IMER), to improve the identification of N-myristoylated peptides by mass spectrometry. The results showed that the MIP monolith exhibited greater selectivity and adsorption capacity compared to previous enrichment materials, providing an efficient means for the protein identification of myristoylated proteomes in complex biological samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
You-Ru Jhou et al.
Summary: An integrated microfluidic platform utilizing real-time reverse-transcription loop-mediated isothermal amplification was developed for detection and quantification of three genes of SARS-CoV-2, providing a useful tool for COVID-19 diagnostics.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Qingfeng Zheng et al.
Summary: Capillary array electrophoresis (CAE) imaging is a cost-effective method that can reveal molecular distribution images in biological tissues with a spatial resolution that can be improved by capillary bore size. The method has the capability to separate charged and non-charged, chiral and nonchiral substances, showing promise for various applications.
Article
Engineering, Environmental
Qingpu Wang et al.
Summary: The study introduces a laminar coflow method for selectively extracting pure Mg(OH)(2) from seawater, which shows improved selectivity compared to conventional methods. By optimizing reaction conditions, the quality and purity of the product can be enhanced. This method has the potential to be broadly applicable in sustainable materials extraction and processing.
ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS
(2022)
Article
Chemistry, Analytical
Leah M. Dignan et al.
Summary: Rotationally-driven lab-on-a-disc (LoaD) microfluidic systems are promising for complex nucleic acid testing, but the lack of on-disc sample preparation has limited their development. This study presents a practical method for inward fluid displacement (IFD) using carbon dioxide gas, allowing for sample preparation on the disc and compatibility with downstream nucleic acid amplification tests. The approach eliminates the need for large architectural features or corrosive chemicals and achieves control over neutralization initiation and pressure containment.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Karen Leirs et al.
Summary: Medical diagnostics is increasingly focusing on low-concentration target detection for early-stage diagnosis. This paper introduces an innovative platform based on digital microfluidics and low-cost disposable chips, enabling ultrasensitive detection of disease-related targets. The potential of the system is demonstrated through thyroid stimulating hormone detection.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Max K. Steinbach et al.
Summary: This study demonstrates a highly sensitive sample preparation workflow using digital microfluidics and high-resolution mass spectrometry for nanoproteomics research.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Tamoghna Saha et al.
Summary: This article presents a wearable system for continuous monitoring of sweat lactate. The system combines a hydrogel for sweat extraction, a paper microfluidic channel for sweat transport, a screen-printed electrochemical lactate sensor, and a custom-built wireless wearable potentiostat system. The system allows for long-term continuous analysis of sweat lactate trends during rest and exercise.
Article
Chemistry, Analytical
Tyler Sano et al.
Summary: Integrated biosensor platforms have become popular due to their ability to combine biosensor processes and functions into a single device, reducing response times. This study demonstrates a complete lab-on-chip system that integrates sample preparation, on-chip optofluidic dye laser, and optical detection, showcasing the potential for rapid and portable point-of-care devices.
Article
Biochemical Research Methods
Laura Heiligenthal et al.
Summary: Microfluidic double-emulsion droplets enable the realization and study of biphasic chemical processes at the nanoliter scale. In this study, double emulsions of a rare type were used to catalyze a lipase-catalyzed reaction, allowing real-time MS analysis. The formation of hydrophilic reaction products within the double-emulsion droplets was successfully verified.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Yongli Chen et al.
Summary: Although liposomes have shown significant success in clinical drug delivery, studying the pharmacokinetics of liposomal nanomedicines remains challenging due to the difficulty in accurately measuring low concentrations of free drug. This study presents a microdialysis-based microfluidic chip interfaced with mass spectrometry to address this issue. By incorporating an effective anti-foulant, PEG 20,0 0 0, the microfluidic chip demonstrated excellent drug extraction and desalting efficiency, providing a promising approach for real-time monitoring of nanomedicine pharmacokinetic profiles.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Chemistry, Multidisciplinary
Xing Xu et al.
Summary: This article introduces a nanoliter-scale single-cell multi-omics sample preparation platform called DMF-DR-seq, based on digital microfluidics. DMF-DR-seq demonstrates improved performance compared to current technology, with lower amplification bias, higher genome-wide coverage in DNA sequencing, and better gene detection ability in RNA sequencing. DMF-DR-seq was used to identify genome variation-induced abnormal transcriptome expression, revealing potential essential genes involved in the pathologic progress. The unique flexibility, sensitivity, and accuracy of DMF-DR-seq suggest its potential utility in deeper multi-omics analysis for inheritance mechanism study in single-cell biology.
Article
Chemistry, Analytical
Uzeyir Dogan et al.
Summary: Pathogen detection is still a challenging issue in public health, especially in food products. Researchers have developed a method using a capillary-driven microfluidic chip and surface-enhanced Raman scattering measurements for rapid detection of Escherichia coli. This method can detect very low concentrations of E. coli within 60 minutes.
Article
Chemistry, Analytical
Jianwei Dong et al.
Summary: A microfluidic magnetic spatial confinement strategy was developed to achieve an ultrasensitive cell immunoassay. By introducing magnetic cell immunocomplexes into microchannels under a permanent magnet, the sensitivity of the assay was enhanced. The strategy showed satisfactory selectivity and reproducibility in model cell detection and enabled the detection of rare cells in practical applications.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Nan Xiang et al.
Summary: In this study, a low-cost, portable, battery-driven microfluidic cell separation instrument was developed for use in resource-limited environments. The instrument integrated various technologies to achieve label-free cell separation and enrichment, and demonstrated excellent performance in terms of separation and enrichment.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Zhuo Chen et al.
Summary: The excessive exploitation of rare earth elements has led to resource depletion and ecological damage. Continuous extraction and recovery of low-concentration REEs at large phase ratios have significant economic and environmental value. The droplet-based microfluidic technique, with the addition of magnetic Janus nanoparticles as emulsifiers, improves extraction efficiency and phase separation speed.
FUNDAMENTAL RESEARCH
(2022)
Article
Biochemical Research Methods
Aseer Intisar et al.
Summary: This study introduces a rapid method for enriching neurons from the mouse embryonic dorsal root ganglion using fully-automated continuous centrifugal microfluidics, achieving effective isolation within 13 minutes. The purified neurons showed improved viability and purity compared to conventional chemical purification methods, highlighting the potential of this automated system to advance neurological research.
Article
Chemistry, Analytical
Abdellah Muhammed et al.
Summary: This study focused on improving sensitivity to trace levels of Cu(II) by preconcentration via coprecipitation, achieving a low detection limit and high recovery rates for spiked water samples. The proposed method is simple, highly sensitive, and cost-effective, making it suitable for analyzing trace levels of Cu(II) in water samples in poorly equipped laboratories.
ANALYTICAL SCIENCES
(2022)
Article
Biochemical Research Methods
Zhixian Zhu et al.
Summary: A polymer microfluidic chip was used for high-throughput and label-free enrichment of malignant tumor cells from pleural and peritoneal effusions. The enriched tumor cells were successfully purified and could be used for rapid drug sensitivity testing. This approach can accelerate cancer diagnosis and guide treatment medication selection.
Article
Chemistry, Multidisciplinary
Aliaa I. Shallan et al.
Summary: The study developed a continuous in situ sampler that can extract particle-free solution from a turbulent high solids content stream without needing to be close to a solid surface. Two applications were demonstrated: monitoring Fe-III in a stirred slurry of mixed ore particles and dissolution profiles of paracetamol tablets with different formulations.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Analytical
Maryam Farahmandi et al.
Summary: A customized microfluidic system was utilized for magnetic solid phase extraction of parabens, achieving efficient extraction and magnetic separation. Magnetite nanoparticles were synthesized and various chemical and instrumental parameters were optimized for characterization and stability of the particles. The method was successfully applied for the extraction of parabens from fruit juice, sunscreen, and urine samples with favorable accuracy and precision.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Jacob Friedrich Hess et al.
Summary: Next generation sequencing is transitioning from a research tool to a method used in diagnostic routine. The complete sequencing workflow includes sample pre-processing, library preparation, sequencing, and bioinformatics analysis. This study presents an automated library preparation method for whole genome sequencing using centrifugal microfluidics, which significantly improves efficiency.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Gilbert L. Nelson et al.
Summary: Microfluidics offer numerous potential applications, including characterization of chemical processes, reaction kinetics studies, sample pretreatment, and portable spectroscopic analyses. In situ characterization of process streams from laboratory-scale and microscale experiments on the same chemical system provides comprehensive understanding of process conditions at different scales. Micro-Raman and micro-UV-vis spectroscopy can determine fundamental parameters with significantly reduced sample size, leading to time, cost, and waste reductions compared to traditional approaches.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Ping Liu et al.
Summary: This study demonstrated a shape-based separation and enrichment method for Saccharomyces cerevisiae using a combination of viscoelastic and Newtonian fluids in a microchannel, showing that the lateral position of yeast cells with varying morphologies depends on the major axis of the cells. The effects of sheath and sample flow rate, PEO concentration, and channel length on the performance of the viscoelastic microfluidic device for yeast enrichment and separation by shape were also investigated, along with the separation efficiency, cell extraction yield, and cell viability after sorting operations.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Wenmei Zhang et al.
Summary: MicroRNAs are important biomarkers that can provide valuable information about diseases and biological processes. An amplification-free method for the simultaneous detection of multiple miRNAs using a pico-HPLC-LIF system has been developed with a zeptomolar limit of detection. This method has been successfully applied to detect cellular miRNA in adenocarcinomic human alveolar basal epithelial cell extracts, enabling the simultaneous detection of mir-182, miR-155, and let-7a expressions.
CHINESE CHEMICAL LETTERS
(2021)
Article
Biophysics
Sifan Meng et al.
Summary: This study developed an innovative 3D surface-enhanced Raman scattering holography sensing strategy for rapid, sensitive, and multiplexed detection of human breast cancer-associated miRNAs. By spatially separating amplified miRNAs on silicon SERS substrates and using different Raman reporters as SERS tags, the detection of miRNAs was completed quickly and with high sensitivity.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biophysics
Hau Van Nguyen et al.
Summary: The portable genetic analyzer presented is equipped with an integrated centrifugal disc for analyzing major feline upper respiratory tract disease pathogens, with the capability of performing loop-mediated isothermal amplification (LAMP) or polymerase chain reaction (PCR) within 1.5 hours.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biochemical Research Methods
Benjamin P. Sullivan et al.
Summary: The translation introduces a paper-based device that uses isotachophoresis to extract and purify nucleic acids from whole blood, providing a simpler sample preparation method for point-of-care NAATs.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yao Shen et al.
Summary: The IPSE chip successfully achieves efficient sample separation and purification at a microscale, rapidly separating the sample solution into two immiscible phases by adding inducers. The system saves time and effort in sample pretreatment, achieves high extraction efficiency, and avoids emulsion formation issues.
Article
Multidisciplinary Sciences
Pierre Garneret et al.
Summary: This study introduces a portable device called COVIDISC for on-site COVID-19 testing, utilizing progress in isothermal amplification and paper microfluidics. The device extracts and amplifies SARS-CoV-2 RNA isothermally using RT-LAMP technology, with detection taking between 20 minutes to 1 hour, and showing no cross-reaction with eight major respiratory viruses currently circulating in Europe. The low cost and simplicity of the device are expected to facilitate widespread dissemination and help manage pandemics.
Article
Chemistry, Multidisciplinary
Hossein Tavassoli et al.
Summary: A label-free method using inertial microfluidics is developed to purify viable cardiomyocytes from mouse neonatal hearts with purities over 90%. The isolated cardiomyocytes retain their identity and function, exhibiting different physico-mechanical properties compared to non-cardiomyocytes. This method could be a valuable tool for advancing the understanding of cardiomyocyte identity and function, with potential benefits for cell therapy and diagnostic applications.
Article
Chemistry, Multidisciplinary
Bo Wang et al.
Summary: A microfluidics-based tripolar system was developed for extracting ionic current in the gas sensing process, compatible with gas chromatography (GC) systems. The system, fabricated with microelectromechanical systems technology, showed higher signal quality than discharge current for ethanol sensing. An analytical model was proposed to explain the ion extraction behavior.
Article
Chemistry, Analytical
Mao Fukuyama et al.
Summary: The technique of deterministically enriching substances in droplets by controlling water transport has the potential to be a powerful pretreatment method for bioassays in droplet microfluidics.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biophysics
Hau Van Nguyen et al.
Summary: The novel centrifugal disc is capable of purifying nucleic acid in a high-throughput manner, processing 30 forensic samples in a single run. It automatically distributes washing solution and elution buffer, featuring a superhydrophobic zigzag-shaped structure for efficient division. The entire process is operated by a portable workstation, enabling quick completion of genetic sex-typing.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Chemistry, Analytical
Ling Xia et al.
Summary: Microfluidics can enhance the performance of surface-enhanced Raman spectroscopy in sample analysis, including reproducibility, selectivity, sensitivity, and speed.
JOURNAL OF SEPARATION SCIENCE
(2021)
Review
Chemistry, Analytical
Mina Alidoust et al.
Summary: The development of chip technology and microfluidics has significantly influenced the field of analytical chemistry, leading to more efficient, cost-effective, user-friendly, and environmentally friendly analytical devices. Microfluidic sample preparative devices have emerged as one of the most important steps in analytical assays, allowing for efficient analyte isolation and easy integration with various detection methods. The extensive development of microfluidic devices is mainly due to their fascinating features such as lower reagent and sample requirements, faster analysis, easier integration and automation, and desirable functionalities through innovative device design.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Ecology
Ryan Wimbles et al.
Summary: This study developed a microfluidic device for genetic analysis of White Rhinoceros dung samples, providing a portable and cost-effective solution for species identification to aid conservation efforts. Optimization of DNA extraction processes and the use of specific amplification reactions allowed for accurate detection results to be obtained quickly.
ECOLOGY AND EVOLUTION
(2021)
Article
Chemistry, Analytical
Jan Leipert et al.
Summary: Digital microfluidics (DMF) is a technology suitable for miniaturized, automated, and multiplexed liquid handling in bioanalytical applications. While its use in LC-MS-based proteomics has been limited to qualitative analyses, the implementation of isobaric peptide labeling on DMF devices has shown promise, especially with the use of maltoside-based detergents. This detergent enables facile droplet movement and is compatible with organic solvents, allowing for efficient isobaric tandem mass tag (TMT) labeling. The newly developed labeling protocol has been successfully applied to multiplexed proteome studies, showing promise for high throughput low cell number proteomics.
ANALYTICAL CHEMISTRY
(2021)
Article
Materials Science, Multidisciplinary
Siwan Park et al.
Summary: E-FLOAT is a novel extractable floating liquid-gel-based organ-on-a-chip that is arrayable, scalable, and can withstand physiologic airflow through microanchors. When combined with a custom airflow system, it efficiently mimics physiological features of airway epithelium. Additionally, E-FLOAT allows standard on-chip analysis as well as complete off-chip analysis, expanding the range of biological assays that can be conducted.
ADVANCED MATERIALS TECHNOLOGIES
(2021)
Article
Chemistry, Multidisciplinary
Seyong Kwon et al.
Summary: A hemolysis-free and highly efficient plasma separation platform has been developed using enhanced diamagnetic repulsion of blood cells. By supplementing blood with superparamagnetic iron oxide nanoparticles, complete removal of blood cells from blood plasma is achieved, allowing for successful collection of plasma without losing plasma proteins, platelets, or exosomes.
Article
Chemistry, Analytical
Xiaojun Li et al.
Summary: The one-step assay using a 3D-printed microfluidic device simplifies enzyme kinetics measurement by eliminating the preparation and handling of multiple solutions. With a highly efficient vibrating mixing method, experiments with different substrate concentrations can be performed by adjusting flow rates, yielding consistent Michaelis constant values. This method offers advantages such as simplicity in fabrication and operation, rapid analysis, low power consumption, and high temporal resolution.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Jiani Yang et al.
Summary: The μMAD technique combines functionalized magnetic beads and a microfluidic device for ultra-sensitive biomarker detection and sample preparation, providing a valuable approach for trace-level biomarker detection in practical applications.
ANALYTICAL CHEMISTRY
(2021)
Article
Energy & Fuels
Farshad Raji et al.
Summary: This research investigated the extraction of bovine serum albumin with aqueous two-phase systems based on polyethylene glycol/sodium citrate using a microfluidic apparatus. The study found that having double interfaces can enhance mass transfer and increase the extraction rate.
CHEMICAL ENGINEERING AND PROCESSING-PROCESS INTENSIFICATION
(2021)
Article
Chemistry, Analytical
Ruben R. G. Soares et al.
Summary: This study demonstrates a highly sensitive Zika virus diagnostic method using padlock probes for isothermal amplification, combined with a microfluidic affinity chromatography enrichment platform. The method showed a positive correlation with RT-qPCR, validating its potential for cost-effective and simple point-of-care viral diagnostics.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Nicholas Grant et al.
Summary: Microfluidics offer advantages to Point of Care devices by reducing reagent use and device size. However, challenges still exist in integrating all supporting hardware and software. This paper demonstrates a self-contained, battery-powered handheld unit capable of operating an EWOD device for DNA extraction and isolation, representing a promising step towards portable microfluidic devices.
Article
Chemistry, Multidisciplinary
Xian Chen et al.
Summary: A novel acoustic streaming tweezers-enabled microfluidic immunoassay was proposed, allowing for probe particles' purification, reaction, and sensing on the same chip under continuous-flow conditions. The high-speed microscale vortexes enable dynamic trapping and washing of the probe particles and enhance the capture efficiency of heterogeneous particle-based immunoassay through active mixing during trapping. This system achieved a limit of detection of 0.2 ng/mL and a dynamic response range from 0.3 to 10 ng/mL for prostate-specific antigen sensing with only 10 μL of sample in less than 15 minutes total assay time, making it suitable for on-site diagnosis applications.
Article
Agricultural Engineering
Soenke Bockmann et al.
Summary: Collecting real-time data on soil nutrients requires the extraction of soil solution into a microfluidic chip using a porous ceramic filter. The extraction rate and water-holding capacity of different soil types were successfully measured using this method, showing promising results for resource-efficient agriculture.
Article
Biochemical Research Methods
Kunpeng Cai et al.
Summary: The study introduces a fully integrated high-throughput microfluidic circulatory fluorescence-activated cell sorting system capable of significantly increasing the purity of rare cells in a short period of time and effectively enriching different types of cells. Through a sequential sorting process and efficient contamination-free design, effective enrichment of clinical rare cells has been achieved.
Article
Biochemical Research Methods
Shubhodeep Paul et al.
Summary: This study introduces microfluidic DNA isolation using the liquid-liquid extraction method in the drop-to-drop format, demonstrating selective extraction of plasmid DNA and optimizing the process using organic dyes as solutes. The results suggest that this method can substitute for magnetic beads steps in standard DNA isolation protocols.
Article
Chemistry, Analytical
Yueqing Shen et al.
Summary: A novel microfluidic chip integrated with magnetic bead enrichment technology and mass spectrometry has been developed, allowing efficient identification of bacterial pathogens with a capture efficiency of up to 90%. The whole procedure takes only 1.5 hours, showing potential for shortening the turnaround time in the clinical diagnosis of bacterial infections.
Article
Biochemical Research Methods
J. -N. Klatt et al.
Summary: The study introduces a centrifugal microfluidic DesaltingDisk for improving reproducibility in sample preparation workflow for proteomic mass spectrometry, especially for applications with limited sample material. The DesaltingDisk automates all liquid handling steps required for peptide desalting, resulting in a more consistent and reliable outcome compared to manual workflows. Evaluation showed reduced variability and improved reproducibility in peptide identification and quantification when using the microfluidic disk.
Article
Biochemical Research Methods
Sa Xiao et al.
Summary: FertDish is a new tool for assisted reproduction technology that allows for rapid selection of high quality sperm, resulting in improvements in DNA fragmentation index, viability, and distribution of sperm with high DNA levels. It is faster, more convenient, and easily adaptable to the clinical workflow compared to traditional methods.
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Food Science & Technology
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