期刊
JOURNAL OF POLYMERS AND THE ENVIRONMENT
卷 -, 期 -, 页码 -出版社
SPRINGER
DOI: 10.1007/s10924-023-03018-2
关键词
Polyhydroxyalkanoates; Cacao mucilage exudate; Bioplastic; Bacillus megaterium
In this study, cacao mucilage exudates (CMEs) were utilized to formulate culture media for the biosynthesis of polyhydroxyalkanoates (PHAs) using a native Bacillus megaterium strain (B2). The strain was successfully adapted to the CMEs-based media and transferred to a batch bioreactor to evaluate PHAs production. The results showed improved productivity and a confirmed structure of polyhydroxybutyrate homopolymer (PHB) for the biopolymer.
Cacao mucilage exudates (CMEs), byproducts of cacao beans fermentation, are effluents rich in sugars and low-molecularweight organic acids. In this study, we utilized CMEs to formulate culture media for the biosynthesis of polyhydroxyalkanoates (PHAs) using a native Bacillus megaterium strain (B2). Our investigation followed the adaptation process of B2 to the CMEs- based media, and we closely monitored the development of the microorganism in terms of consumption of acids and sugars. Upon successful adaptation to the CMEs-based media, we transferred the strain to a 4- L batch bioreactor to evaluate the impact of operational variables on PHAs production. We examined the microorganisms' yield, productivity, and growth kinetics using the CME- based media. Our results showed a product yield of 0.98 g PHA/g carbon source, with an accumulation percentage of 57% and a productivity increase of 33% compared to PHA produced from residual glycerol using the same microorganism. The biopolymer's structure was confirmed to be of a polyhydroxybutyrate homopolymer (PHB) type using various molecular characterization techniques, including Matrix-Assisted Laser Desorption/Ionization mass spectrometry (MALDI-TOF-MS), infrared spectroscopy (IR), elemental composition (EC), ultraviolet-visible spectroscopy (UV-VIS), and thermal analysis (TGA, DSC).
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