期刊
MOLECULAR BIOLOGY AND EVOLUTION
卷 33, 期 11, 页码 2781-2800出版社
OXFORD UNIV PRESS
DOI: 10.1093/molbev/msw141
关键词
allopolyploid; homeolog; hyperaccumulation; heavy metal; selective sweep; transcriptomics
资金
- Swiss National Science Foundation
- University Research Priority Program of Evolution in Action of the University of Zurich [16H06469, 26113709]
- Indo-Swiss Collaboration in Biotechnology
- Special Coordination Funds for Promoting Science and Technology
- MEXT Japan
- Inamori Foundation research
- Japan Society for the Promotion of Science [2277023]
- Research and Education Funding for Japanese Alps Inter-Universities Cooperative Project, MEXT, Japan
- Grants-in-Aid for Scientific Research [16H06469, 15H02670] Funding Source: KAKEN
Allopolyploidization combines parental genomes and often confers broader species distribution. However, little is known about parentally transmitted gene expression underlying quantitative traits following allopolyploidization because of the complexity of polyploid genomes. The allopolyploid species Arabidopsis kamchatica is a natural hybrid of the zinc hyperaccumulator Arabidopsis halleri and of the nonaccumulator Arabidopsis lyrata. We found that A. kamchatica retained the ability to hyperaccumulate zinc from A. halleri and grows in soils with both low and high metal content. Hyperaccumulation of zinc by A. kamchatica was reduced to about half of A. halleri, but is 10-fold greater than A. lyrata. Homeologs derived from A. halleri had significantly higher levels of expression of genes such as HEAVY METAL ATPASE4 (HMA4), METAL TRANSPORTER PROTEIN1 and other metal ion transporters than those derived from A. lyrata, which suggests cis-regulatory differences. A. kamchatica has on average about half the expression of these genes compared with A. halleri due to fixed heterozygosity inherent in allopolyploids. Zinc treatment significantly changed the ratios of expression of 1% of homeologous pairs, including genes putatively involved in metal homeostasis. Resequencing data showed a significant reduction in genetic diversity over a large genomic region (290 kb) surrounding the HMA4 locus derived from the A. halleri parent compared with the syntenic A. lyrata-derived region, which suggests different evolutionary histories. We also estimated that three A. halleri-derived HMA4 copies are present in A. kamchatica. Our findings support a transcriptomic model in which environment-related transcriptional patterns of both parents are conserved but attenuated in the allopolyploids.
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