Silencing suppressor of cytokine signaling 3 induces apoptosis and activates the p-STAT3/NF-κB pathway in hypoxic cultivated H9c2 cells

Suppressor of cytokine signaling 3 (SOCS3) plays a significant role in the process of myocardial adaptation to chronic hypoxia. SOCS3 finely regulates cell signaling cross-talk that occurs between NF-kappa B and STAT3 during the compensatory protective response. However, the role and mechanism of SOCS3 in hypoxic cardiomyocytes are not fully understood. In the study, we investigated the effect of SOCS3 on the p65 and STAT3 signaling pathways and further examined the potential molecular mechanism involved in regulating apoptosis. Our data showed that SOCS3 silencing could upregulate Ac-p65, p-p65, and p-STAT3 expression in nuclear extracts of H9c2 cells that received hypoxic treatment for 24, 48, and 72 h. SOCS3 silencing also remarkably increased the DNA-binding activity of the p65 motif in hypoxic cultivated H9c2 cells. We also found that SOCS3 knockdown increased cleaved-caspase-3, Bax, and PUMA expression and decreased cleaved PARP and Bcl-2 in expression in hypoxic H9c2 cells. Silencing of SOCS3 caused an increase in LDH leakage from injured cardiomyocytes and reduced cell viability under conditions of hypoxic stress. Furthermore, SOCS3 silencing enhanced the apoptosis of H9c2 cells at 72 h of hypoxia. These findings suggest that knockdown of SOCS3 leads to excessive activation of the NF-kappa B pathway, which, in turn, might promote apoptosis under conditions of chronic hypoxia.Graphical AbstractDiagram showing the mechanism of SOCS3 in hypoxic cardiomyocytes. Silencing SOCS3 could induce the apoptosis of H9c2 cells by regulating the apoptosis-associated gene expression, which may be accomplished by activation of the p-STAT3/NF-kappa B signaling pathway and rely on hypoxia condition.

Automated summary

In hypoxic cardiomyocytes, silencing of SOCS3 induces apoptosis of H9c2 cells by regulating apoptosis-associated gene expression, possibly through activation of the p-STAT3/NF-kappa B signaling pathway under hypoxic conditions.