Effects of temperature and nutrients on microscopic stages of the bull kelp (Nereocystis luetkeana, Phaeophyceae)

Warming ocean temperatures have been linked to kelp forest declines worldwide, and elevated temperatures can act synergistically with other local stressors to exacerbate kelp loss. The bull kelp Nereocystis luetkeana is the primary canopy-forming kelp species in the Salish Sea, where it is declining in areas with elevated summer water temperatures and low nutrient concentrations. To determine the interactive effects of these two stressors on microscopic stages of N. luetkeana, we cultured gametophytes and microscopic sporophytes from seven different Salish Sea populations across seven different temperatures (10-22?) and two nitrogen concentrations. The thermal tolerance of microscopic gametophytes and sporophytes was similar across populations, and high temperatures were more stressful than low nitrogen levels. Additional nitrogen did not improve gametophyte or sporophyte survival at high temperatures. Gametophyte densities were highest between 10 and 16? and declined sharply at 18?, and temperatures of 20 and 22? were lethal. The window for successful sporophyte production was narrower, peaking at 10-14?. Across all populations, the warmest temperature at which sporophytes were produced was 16 or 18?, but sporophyte densities were 78% lower at 16? and 95% lower at 18? compared to cooler temperatures. In the field, bottom temperatures revealed that the thermal limits of gametophyte growth (18?) and sporophyte production (16-18?) were reached during the summer at multiple sites. Prolonged exposure of bull kelp gametophytes to temperatures of 16? and above could limit reproduction, and therefore recruitment, of adult kelp sporophytes.

Automated summary

Warming ocean temperatures and low nutrient concentrations have led to declines in bull kelp populations in the Salish Sea. In laboratory experiments, high temperatures had a more negative impact on microscopic stages of bull kelp compared to low nitrogen levels. Gametophytes were most abundant between 10 and 16℃, while sporophyte production peaked at 10-14℃. Field observations confirmed that the thermal limits for gametophyte growth (18℃) and sporophyte production (16-18℃) were reached during the summer. Caution should be taken to prevent prolonged exposure of bull kelp gametophytes to temperatures above 16℃, as it could limit reproduction and recruitment of adult sporophytes.