期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 231, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jpba.2023.115414
关键词
In vitro digestion; Acetyl isomerization; Cardiovascular health care; Saponins; Flavonoids
Radix Astragali (RA) is a commonly used Asian herbal therapy or food supply with astragalosides and flavonoids as its major components. This study investigated the bioaccessibility of these compounds during the process of oral digestion and examined their effects on intracellular reactive oxygen species levels in a human arterial endothelial cells model. The results indicate that the acetyl biotransformation of RA in the small intestine directly affects the response to oxidative stress, providing insights into the multi-component action of oral RA in cardiovascular health care.
Radix Astragali (RA) is commonly used in Asian herbal therapy or food supply, and astragalosides and flavonoids are its major components with diverse pharmaceutical effects. To provide new information on the potential cardiovascular benefits of RA administered orally, the bioaccessibility of these compounds with relevant in vitro digestion parameters was determined for four digestion phases (oral, gastric, small and large intestines) by ultrahigh-performance liquid chromatography quadrupole time-of-flight-mass spectrometry (UPLC-Q-TOF/MS). Meanwhile, we compared the effects of digestion products on advanced glycation end products (AGEs)-induced intracellular reactive oxygen species (ROS) levels in a human arterial endothelial cells (HAECs) model, and studied the potential of RA against oxidative stress-related cardiovascular disease. The changes of saponins and flavonoids composition and antioxidant activity after digestion in intestines were mainly due to the astragaloside IV (AS-IV) biosynthesis involving saponins acetyl isomerization and deacetylation, and the flavonoid glycosides converted to aglycone by deglycosylation processes. All these results suggest that acetyl biotransformation of RA in small intestine directly influenced the response to oxidative stress, and might provide a reference for elucidation of the multi-component action after oral RA in cardiovascular health care.
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