期刊
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
卷 236, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jpba.2023.115705
关键词
Silver nanoparticles; Localized surface plasmon resonance; Colorimetric sensor; Pharmaceutical Formulations; Glucosamine
This study developed a novel method for synthesizing silver nanoparticles using glucosamine as a reducing agent, and applied the nanoparticles for colorimetric detection and quantitative determination of the non-chromophoric drug, glucosamine. The method showed good sensitivity and accuracy.
The purpose of this study is to develop a novel method for synthesizing silver nanoparticles using glucosamine as reducing agent and to utilize the developed method for colorimetric detection and quantitative determination of the non-chromophoric drug, glucosamine. Silver nanoparticles are prepared by reducing 0.02 mol/L silver nitrate by glucosamine in 0.075 mol/L ammonia and stabilizing the nanoparticles with 0.1% polyvinylpyrrolidone and the mixture is heated at 90 degrees C for 5 min. The prepared silver nanoparticles dispersed in water exhibit a bright yellow color due to a localized surface plasmon resonance band at 412 nm. The principle of glucosamine sensing is based on measuring the intensity of the surface plasmon resonance band at 412 nm which is directly proportional to the concentration of glucosamine with a linearity range (1 - 9 mu g/mL), limit of detection 0.33 mu g/mL and limit of quantitation 1.0 mu g/mL. The proposed method was validated according to the ICH guidelines, and it was found to be accurate, precise, selective, and robust. The method was applied for determination of glucosamine in Joflex (R) capsules using the standard addition approach with mean % recovery +/- standard deviation of 100.077 +/- 1.786. The method is simple, rapid, and cost-effective and can be used for determination of glucosamine in bulk and in its pharmaceutical preparations.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据