4.6 Article

Benfluorex metabolism complemented by electrochemistry-mass spectrometry

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ELSEVIER
DOI: 10.1016/j.jpba.2023.115626

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Electrochemistry; Mass spectrometry; Benfluorex; Norfenfluramine; Metabolism

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This study investigates the metabolites of the hypolipidemic and hypoglycemic drug benfluorex, and finds that its metabolite norfenfluramine may be associated with valvular heart disease and pulmonary arterial hypertension. Electrochemical metabolism mimicry combined with mass spectrometry is used for rapid screening of metabolites, which could have predicted the potential adverse reactions of benfluorex. This highlights the importance of electrochemical metabolism mimicry for drug safety evaluation.
The hypolipidemic and hypoglycemic drug benfluorex was widely applied to treat type 2 diabetes mellitus and metabolic syndrome in overweight patients since 1976. However, benfluorex was connected to multiple cases of valvular heart disease and pulmonary arterial hypertension later on. Similar adverse drug reactions were previously found to be associated to the structurally related drug fenfluramine, which was attributed to the formation of its N-deethylated metabolite norfenfluramine. Even though norfenfluramine was known to be a common metabolite of fenfluramine and benfluorex, only fenfluramine was withdrawn from European and United States markets in 1997 while benfluorex remained available until 2009. In this work, the metabolism of benfluorex is simulated by an online hyphenation of electrochemistry and mass spectrometry and the observed transformation products are further characterized using liquid chromatography and high-resolution tandem mass spectrometry. Using this approach, norfenfluramine is found to be the main electrochemical transformation product of benfluorex. Considering the knowledge about norfenfluramine toxicity, rapid metabolite screening using electrochemistry hyphenated to mass spectrometry could have been used to predict the potential of benfluorex for adverse drug reactions early on, showcasing the value of electrochemical metabolism mimicry for rapid drug safety evaluation.

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