期刊
MOLECULAR AND CELLULAR ENDOCRINOLOGY
卷 431, 期 C, 页码 88-100出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2016.05.003
关键词
NMR metabolomics; Mouse; Obesity; Type 2 diabetes mellitus; Urine; Antidiabetic treatment
资金
- Grant Agency of the Czech Republic [GA13-14105S]
- Operational Program Prague - Competitiveness [CZ.2.16/3.1.00/24023]
- specific university research (MSMT) of the Ministry of Education, Youth and Sports of the Czech Republic [20/2015]
- [LO1509]
Metformin, vildagliptin and their combination are widely used for the treatment of diabetes, but little is known about the metabolic responses to these treatments. In the present study, NMR-based metabolomics was applied to detect changes in the urinary metabolomic profile of a mouse model of diet induced obesity in response to these treatments. Additionally, standard biochemical parameters and the expression of enzymes involved in glucose and fat metabolism were monitored. Significant correlations were observed between several metabolites (e.g., N-carbamoyl-beta-alanine, N1-methyl-4-pyridone-3-carboxamide, N1-methyl-2-pyridone-5-carboxamide, glucose, 3-indoxyl sulfate, dimethylglycine and several acylglycines) and the area under the curve of glucose concentrations during the oral glucose tolerance test. The present study is the first to present N-carbamoyl-beta-alanine as a potential marker of type 2 diabetes mellitus and consequently to demonstrate the efficacies of the applied antidiabetic interventions. Moreover, the elevated acetate level observed after vildagliptin administration might reflect increased fatty acid oxidation. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
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