4.7 Article

Evolutionary Engineering a Larger Porin Using a Loop-to-Hairpin Mechanism

期刊

JOURNAL OF MOLECULAR BIOLOGY
卷 435, 期 22, 页码 -

出版社

ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2023.168292

关键词

outer membrane proteins; b-barrel; loop-to-hairpin; molecular evolution; neisserial porin

向作者/读者索取更多资源

In protein evolution, diversification is often driven by genetic duplication. However, a computational study suggests that another evolutionary mechanism involving a transition from loops to hairpins can lead to an increase in the number of strands in outer membrane proteins.
In protein evolution, diversification is generally driven by genetic duplication. The hallmarks of this mech-anism are visible in the repeating topology of various proteins. In outer membrane (3-barrels, duplication is visible with (3-hairpins as the repeating unit of the barrel. In contrast to the overall use of duplication in diversification, a computational study hypothesized evolutionary mechanisms other than hairpin duplica-tions leading to increases in the number of strands in outer membrane (3-barrels. Specifically, the topology of some 16-and 18-stranded (3-barrels appear to have evolved through a loop to (3-hairpin transition. Here we test this novel evolutionary mechanism by creating a chimeric protein from an 18-stranded (3-barrel and an evolutionarily related 16-stranded (3-barrel. The chimeric combination of the two was created by replacing loop L3 of the 16-stranded barrel with the sequentially matched transmembrane (3-hairpin region of the 18-stranded barrel. We find the resulting chimeric protein is stable and has characteristics of increased strand number. This study provides the first experimental evidence supporting the evolution through a loop to (3-hairpin transition.(c) 2023 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://crea-tivecommons.org/licenses/by-nc-nd/4.0/).

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据