Changes of gut mycobiota in the third trimester of pregnant women with preeclampsia

Objective Studies recently acknowledged that the fungal community in the gut plays an important role in many inflammatory diseases of noninfectious origin. but the role of gut fungi in the pathogenesis of preeclampsia (PE) remains unknown. Methods We performed a case-case-control study to compare the gut mycobiota of PE, pregnancy with chronic hypertension (PCH), and the normal group (Normal pregnancy group without any underlying disease) by internal transcribed spacer sequencing. In addition, LC/MS was used to explore the relationship between the fecal metabolites and gut mycobiota. Results Compared with the PCH and the normal group, & alpha; diversity (represented the species abundance in a single sample) of mycobiota were lower in the PE group, but there was no statistically significant difference among these three groups. However, Linear discriminant analysis Effect Size (LEfSe) analysis found 3 differentially abundant fungal taxa in PE group when compared with the normal group. The gut metabolites of PE patients were significantly different from PCH and the normal group. Choline metabolism molecule glycerophosphocholine was the most discriminant metabolite between PE and the normal group. Correlation analysis found that Candida spp.was positively correlated with glycerophosphocholine which increased in PE. Conclusion We found that gut mycobiota changed in the third trimester of pregnant women with preeclampsia.

Automated summary

Recent studies have shown that gut fungi play a crucial role in many noninfectious inflammatory diseases, but their role in the pathogenesis of preeclampsia remains unclear. In this study, the gut mycobiota of preeclampsia, pregnancy with chronic hypertension, and normal pregnancy groups were compared using internal transcribed spacer sequencing. The results revealed changes in the gut mycobiota in the third trimester of pregnant women with preeclampsia. Additionally, the correlation analysis indicated a positive correlation between Candida spp. and glycerophosphocholine, which was significantly increased in preeclampsia.