期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 455, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jhazmat.2023.131540
关键词
Crotonylation; Dibutyl phthalate; MSH6; NHEJ; Homologous recombination
Maternal exposure to DBP resulted in significant abnormalities during meiotic prophase I in fetal ovaries, including delayed progression, impaired DNA damage response, uncoupled localization of DMC1 and RAD51, and decreased homologous recombination (HR). DBP negatively regulated lysine crotonylation (Kcr) of MSH6. F2 female mice exhibited premature follicular activation, precocious puberty, and anxiety-like behaviors.
Homologous recombination (HR) during early oogenesis repairs programmed double-strand breaks (DSBs) to ensure female fertility and offspring health. The exposure of fetal ovaries to endocrine disrupting chemicals (EDCs) can cause reproductive disorders in the adulthood. The EDC dibutyl phthalate (DBP) is widely distributed in flexible plastic products, leading to ubiquitous human exposure. Here, we report that maternal exposure to DBP caused gross aberrations in meiotic prophase I of fetal oocytes, including delayed progression, impaired DNA damage response, uncoupled localization of DMC1 and RAD51, and decreased HR. However, programmed DSBs were efficiently repaired. DBP exposure negatively regulated lysine crotonylation (Kcr) of MSH6. Similar meiotic defects were observed in fetal ovaries with targeted disruption of Msh6, and mutation of K544cr of MSH6 impaired its association with Ku70, thereby promoting non-homologous end joining (NHEJ) and inhibiting HR. Unlike mature F1 females, F2 female mice exhibited premature follicular activation, precocious puberty, and anxiety-like behaviors. Therefore, DBP can influence early meiotic events, and Kcr of MSH6 may regulate preferential induction of HR or NHEJ for DNA repair during meiosis.
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