Bioremediation of PBDEs and heavy metals co-contaminated soil in e-waste dismantling sites by Pseudomonas plecoglossicida assisted with biochar

Biochar-assisted microbial remediation has been proposed as a promising strategy to eliminate environmental pollutants. However, studies on this strategy used in the remediation of persistent organic pollutants and heavy metals co-contaminated soil are lacking, and the effect of the combined incorporation of biochar and inoculant on the assembly, functions, and microbial interactions of soil microbiomes are unclear. Here, we studied 2,2 & PRIME;,4,4 & PRIME;tetrabrominated diphenyl ether (BDE-47) degradation and heavy metal immobilization by and biochar-based bacterial inoculant (BC/PP) in an e-waste contaminated soil, and corresponding microbial regulation mechanisms. Results showed that BC/PP addition was more effective in reducing Cu and Pb availability and degrading BDE-47 than inoculant alone. Notably, BC/PP facilitated bound-residue formation of BDE-47, reducing the ecological risk of residual BDE-47. Meanwhile, microbial carbon metabolism and enzyme activities (related to C-, N-, and P- cycles) were enhanced in soil amended with BC/PP. Importantly, biochar played a crucial role in inoculant colonization, community assembly processes, and microbiome multifunction. In the presence of biochar, positive interactions in co-occurrence networks of the bacterial community were more frequent, and higher network stability and more keystone taxa were observed (including potential degraders). These findings provide a promising strategy for decontaminating complex-polluted environments and recovering soil ecological functions.

Automated summary

This study investigated the use of biochar-assisted microbial remediation for eliminating environmental pollutants and the effect of biochar and inoculant on soil microbiomes. The results showed that the combined application of biochar and inoculant was more effective in reducing heavy metal availability and degrading organic pollutants than inoculant alone. Additionally, biochar played a crucial role in promoting the colonization of inoculant and enhancing microbial interactions and functions.