期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 456, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.jhazmat.2023.131656
关键词
Environmental DNA; EDNA; Metabarcoding; Biocontamination; DWTP
In this study, environmental DNA (eDNA) metabarcoding was used to monitor invertebrate outbreaks at different stages of drinking water treatment. The composition of invertebrate eDNA communities reflected the source water communities in earlier stages, but some dominant taxa were introduced during purification and eliminated in later stages. The study also assessed the detection/quantification limits and read capacity of eDNA metabarcoding for biocontamination surveillance in drinking water treatment plants (DWTPs). A novel eDNA-based approach for sensitive and efficient surveillance of invertebrate outbreaks in DWTPs was proposed.
A clean and adequate supply of drinking water is essential to life and good health. However, despite the risk of biologically derived contamination of drinking water, monitoring of invertebrate outbreaks has relied primarily on naked-eye inspections that are prone to errors. In this study, we applied environmental DNA (eDNA) metabarcoding as a biomonitoring tool at seven different stages of drinking water treatment, from prefiltration to release from household faucets. While the composition of invertebrate eDNA communities reflected the communities of the source water in earlier stages of the treatment, several predominant invertebrate taxa (e.g., rotifer) were shown to be introduced during purification, but most were eliminated in later treatment stages. In addition, the limit of detection/quantification of PCR assay and read capacity of high-throughput sequencing was assessed with further microcosm experiments to estimate the applicability eDNA metabarcoding to the biocontamination surveillance in drinking water treatment plants (DWTPs). Here we propose a novel eDNA-based approach for sensitive and efficient surveillance of invertebrate outbreaks in DWTPs.
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