4.4 Article

Open reading frames M12/M13 jointly contribute to MHV-68 latency

期刊

JOURNAL OF GENERAL VIROLOGY
卷 104, 期 8, 页码 -

出版社

MICROBIOLOGY SOC
DOI: 10.1099/jgv.0.001880

关键词

gammaherpesvirus; ORF M13; ORF M12; MHV-68

向作者/读者索取更多资源

The function of MHV-68-specific ORFs M12 and M13 have been investigated through construction and analysis of recombinant MHV-68 with mutations. It was found that M12 and M13 genes play an important role during latency in vivo.
Murine gammaherpesvirus 68 (MHV- 68), a widely used small-animal model for the analysis of gammaherpesvirus patho-genesis, encodes the MHV- 68-specific ORFs M12 and M13. The function of M12 and M13 has not been investigated so far. Therefore, we constructed and analysed recombinant MHV- 68 with mutations in either M12, M13 or M12/M13. Both the M12 and M13 mutants did not display any phenotype in vitro or in vivo. However, although the M12/13 double mutant showed similar lytic growth in fibroblasts in vitro and in the lungs of infected mice as wild -type MHV- 68, it was significantly attenu-ated in vivo during latency. This phenotype was completely restored in a revertant of the M12/13 double mutant. Thus, it appears that M12 and M13 might have redundant functions that are only revealed if both genes are lacking. The observa-tion that M12/13 have a function during latency not only contributes to the further understanding of the pathogenesis of MHV- 68 infection but might also be of interest considering that M12/13 are located at a genomic position similar to that of LMP2A and K15. The latter are important proteins of their respective human gammaherpesviruses EBV and KSHV that contribute to cellular survival, cell activation and proliferation, which was deduced from in vitro studies.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.4
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据