4.7 Article

A novel natural variation in the promoter of GmCHX1 regulates conditional gene expression to improve salt tolerance in soybean

期刊

JOURNAL OF EXPERIMENTAL BOTANY
卷 -, 期 -, 页码 -

出版社

OXFORD UNIV PRESS
DOI: 10.1093/jxb/erad404

关键词

cis-element; GmCHX1; QTL mapping; salt tolerance; soybean

向作者/读者索取更多资源

Identification and characterization of soybean germplasm and gene(s)/allele(s) for salt tolerance is crucial for developing improved varieties for saline soils. This study identified four salt-tolerant soybean lines with new gene(s) or new functional allele(s) of GmCHX1. QTL mapping revealed two major salt tolerance loci, including a novel locus associated with leaf scorch score, and variations in the promoter region of GmCHX1 were found to regulate its salt-inducible expression. These findings suggest the potential use of gene-editing techniques in improving salt tolerance in soybean.
Identification and characterization of soybean germplasm and gene(s)/allele(s) for salt tolerance is an effective way to develop improved varieties for saline soils. Previous studies identified GmCHX1 (Glyma03g32900) as a major salt tolerance gene in soybean, and two main functional variations were found in the promoter region (148/150 bp insertion) and the third exon with a retrotransposon insertion (3.78 kb). In the current study, we identified four salt-tolerant soybean lines, including PI 483460B (Glycine soja), carrying the previously identified salt-sensitive variations at GmCHX1, suggesting new gene(s) or new functional allele(s) of GmCHX1 in these soybean lines. Subsequently, we conducted quantitative trait locus (QTL) mapping in a recombinant-inbred line population (Williams 82 (salt-sensitive) x PI 483460B) to identify the new salt tolerance loci/alleles. A new locus, qSalt_Gm18, was mapped on chromosome 18 associated with leaf scorch score. Another major QTL, qSalt_Gm03, was identified to be associated with chlorophyll content ratio and leaf scorch score in the same chromosomal region of GmCHX1 on chromosome 3. Novel variations in a STRE (stress response element) cis-element in the promoter region of GmCHX1 were found to regulate the salt-inducible expression of the gene in these four newly identified salt-tolerant lines including PI 483460B. This new allele of GmCHX1 with salt-inducible expression pattern provides an energy cost efficient (conditional gene expression) strategy to protect soybean yield in saline soils without yield penalty under non-stress conditions. Our results suggest that there might be no other major salt tolerance locus similar to GmCHX1 in soybean germplasm, and further improvement of salt tolerance in soybean may rely on gene-editing techniques instead of looking for natural variations. A novel variation in GmCHX1 promoter regulates conditional gene expression to improve salt tolerance for an energy-cost efficient strategy to protect soybean yield in saline soils.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据