期刊
JOURNAL OF ENVIRONMENTAL MANAGEMENT
卷 342, 期 -, 页码 -出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jenvman.2023.118281
关键词
Clostridium thermocellum; Consolidated bio-saccharification; Geobacillus stearothermophilus; Lignocellulose; PLA
In this study, a thermophilic strain Geobacillus stearothermophilus 2H-3 was successfully used for the production of L-(+) lactic acid (LA) from various agricultural wastes through a consolidated bio-saccharification (CBS) process. The one-pot successive fermentation process achieved high optical purity (99.5%), titer (51.36 g/L), and yield (0.74 g/gbiomass) of LA. This study provides a promising strategy for LA production from lignocellulose through CBS and 2H-3 fermentation integration.
The production of lactic acid (LA) from agricultural wastes attracts great attention because of the sustainability and abundance of lignocellulosic feedstocks, as well as the increasing demand for biodegradable polylactic acid. In this study, we isolated a thermophilic strain Geobacillus stearothermophilus 2H-3 for use in robust production of L-(+)LA under the optimal conditions of 60 degrees C, pH 6.5, which were consistent with the whole-cell-based consolidated bio-saccharification (CBS) process. Sugar-rich CBS hydrolysates derived from various agricultural wastes, including corn stover, corncob residue, and wheat straw, were used as the carbon sources for 2H-3 fermentation by directly inoculating 2H-3 cells into the CBS system, without intermediate sterilization, nutrient supplementation, or adjustment of fermentation conditions. Thus, we successfully combined two whole-cell-based steps into a one-pot successive fermentation process to efficiently produce LA with high optical purity (99.5%), titer (51.36 g/L), and yield (0.74 g/gbiomass). This study provides a promising strategy for LA production from lignocellulose through CBS and 2H-3 fermentation integration.
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