An electrochemical biosensor based on CuFe PBA/MoS2 nanocomposites for stable and sensitive detection of hydrogen peroxide and carcinoembryonic antigen

An increasing number of electrochemical biosensors have been constructed to detect single bioactive sub-stances with high sensitivity, but not multiple bioactive substances with different properties. Therefore, we incorporated Cu and Fe elements into Prussian blue to form Prussian blue analogues (PBA) to improve the elec-trochemical catalytic activity of Prussian blue. At the same time, MoS2 is used as the material substrate to increase the electrochemical active site of the composite. We have constructed a universal electrochemical sensing platform using composite materials (CuFe PBA/MoS2) as electrode modification materials for detecting two different types of representative cancer biomarkers, hydrogen peroxide (H2O2) and carcinoembryonic anti-gen (CEA). The universal electrochemical biosensor showed a significant linear response to both H2O2 and CEA with the lowest detection limits of 0.23 & mu;M and 0.01 ng mL-1, respectively, and it had high selectivity, repro-ducibility, and stability. The universal electrochemical biosensor is successfully applied to detect H2O2 released from human breast cancer (MCF-7) cells and CEA expressed on the surface of human cervical cancer (HeLa) cells. The developed biosensor has potential in the dynamic detection of the flux of H2O2 and the expression level of CEA from living cells. The high sensitivity of this universal sensor provides a novel strategy for simul-taneously detecting multiple cancer biomarkers.

Automated summary

A new electrochemical biosensor platform has been developed using composite materials (CuFe PBA/MoS2) as electrode modification materials, which can simultaneously detect multiple cancer biomarkers such as H2O2 and CEA with high sensitivity, selectivity, and stability.