Leaf nitrogen affects photosynthesis and water use efficiency similarly in nitrogen-fixing and non-fixing trees

Nitrogen (N)-fixing trees are thought to break a basic rule of leaf economics: higher leaf N concentrations do not translate into higher rates of carbon assimilation. Understanding how leaf N affects photosynthesis and water use efficiency (WUE) in this ecologically important group is critical.We grew six N-fixing and four non-fixing tree species for 4-5 years at four fertilization treatments in field experiments in temperate and tropical regions to assess how functional type (N fixer vs. non-fixer) and N limitation affected leaf N and how leaf N affected light-saturated photosynthesis (Asat), stomatal conductance (gsw) and WUE (WUEi and & delta;13C).Asat, WUEi and & delta;13C, but not gsw, increased with higher leaf N. Surprisingly, N-fixing and non-fixing trees displayed similar scaling between leaf N and these physiological variables, and this finding was supported by reanalysis of a global dataset. N fixers generally had higher leaf N than non-fixers, even when non-fixers were not N-limited at the leaf level. Leaf-level N limitation did not alter the relationship of Asat, gsw, WUEi and & delta;13C with leaf N, although it did affect the photosynthetic N use efficiency. Higher WUE was associated with higher productivity, whereas higher Asat was not.Synthesis: The ecological success of N-fixing trees depends on the effect of leaf N on carbon gain and water loss. Using a field fertilization experiment and reanalysis of a global dataset, we show that high leaf-level photosynthesis and WUE in N fixers stems from their higher average leaf N, rather than a difference between N fixers and non-fixers in the scaling of photosynthesis and WUE with leaf N. By clarifying the mechanism by which N fixers achieve and benefit from high WUE, our results further the understanding of global N fixer distributions. The ecological success of N-fixing trees depends on the effect of leaf N on carbon gain and water loss. Using a field fertilization experiment and reanalysis of a global dataset, we show that high leaf-level photosynthesis and water use efficiency (WUE) in N fixers stems from their higher average leaf N, rather than a difference between N fixers and non-fixers in the scaling of photosynthesis and WUE with leaf N. By clarifying the mechanism by which N fixers achieve and benefit from high WUE, our results further the understanding of global N fixer distributions.image

Automated summary

Nitrogen (N)-fixing trees challenge the basic rule of leaf economics by showing that higher leaf nitrogen concentrations do not necessarily result in higher rates of photosynthesis. Understanding how leaf nitrogen affects photosynthesis and water use efficiency is crucial for this ecologically important group.