期刊
JOURNAL OF DENTAL RESEARCH
卷 -, 期 -, 页码 -出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/00220345231186394
关键词
Sjogren's syndrome; Sjogren syndrome; hepatitis d virus; hepatitis delta virus; hepatitis b virus; non-communicable chronic disease
This study found that hepatitis delta virus (HDV) was detected in the minor salivary gland (MSG) tissue of Sjogren's disease (SjD) patients without hepatitis B virus (HBV) coinfection. The expression of HDV antigen (HDAg) can trigger an SjD-like phenotype in vivo. HDAg was detected in different cell types and subcellular locations in the MSG, and HDV genomic RNA was localized in the MSG nuclei. The intensity of HDAg showed negative correlations with lymphocytic inflammation and certain autoimmune disease comorbidities in SjD patients.
Hepatitis delta virus (HDV) has been detected in the minor salivary gland (MSG) tissue of Sjogren's disease (SjD) patients in the absence of a hepatitis B virus (HBV) coinfection. Previous research has shown that HDV antigen (HDAg) expression can trigger an SjD-like phenotype in vivo, demonstrating a potential cause-and-effect relationship. We hypothesize that if HDV plays a role in the development of SjD, then HDV profiles may be correlated with disease manifestations. This retrospective study characterized HDV in a cohort of 48 SjD MSG samples collected between 2014 and 2021. Analyses of HDAg expression, including cell type and subcellular localization, in situ hybridization of HDV RNA, and comparative analyses with associated SjD and viral hepatitis clinical features, were conducted. HDAg was detected in MSG acinar, ductal, myoepithelial, and adipose cells and localized with the nuclei, cytoplasm, and mitochondria. In situ hybridization detected HDV genomic RNA localization in the MSG nuclei. A significant negative correlation was found between HDAg intensity and focal lymphocytic inflammation and in patients with both anti-SSA/Ro-52 and anti-SSA/Ro-60. In analyzing autoimmune disease comorbidities with SjD, it was found that SjD patients diagnosed with autoimmune thyroiditis and/or hypothyroidism were significantly more represented in the high HDAg intensity group compared to the negative and moderate HDAg intensity groups. No significant associations were detected between MSG-localized HDAg and liver enzymes or an evident HBV coinfection. This study has further confirmed that there is a nonhepatic reservoir for chronic HDV persistence in SjD-affected salivary gland tissue in a third independent SjD patient cohort. In addition, this study describes the unique colocalization of HDAg with mitochondria. The detection of HDV antigen and sequence within SjD-affected salivary gland tissue, and in the absence of an evident current or past HBV coinfection, warrants further investigation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据