Multifunctional self-assembled polymeric nanoprobes for FRET-based ratiometric detection of mitochondrial H2O2 in living cells

A ratiometric, multifunctional nanoprobe was prepared consisting of a self-assembled polymeric micelle as the carrier, tetraphenylethene (TPE) as the donor, fluorescent boronate as the H2O2-responsive acceptor, and triphenylphosphonium as a mitochondria-targeted moiety. The assembled nanoparticles could detect both exogenous and endogenous mitochondrial H2O2 changes in living cells.