4.6 Article

Evolution of anthocyanin content during grape ripening and characterization of the phenolic profile of the resulting wine by comprehensive two-dimensional liquid chromatography

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JOURNAL OF CHROMATOGRAPHY A
卷 1704, 期 -, 页码 -

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ELSEVIER
DOI: 10.1016/j.chroma.2023.464131

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Phenolic compounds; Grape; Wine; Two-dimensional liquid chromatography; LC x LC

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In this study, a new method based on comprehensive two-dimensional liquid chromatography coupled to a diode array detector and tandem mass spectrometry was developed to obtain the phenolic profile of Malbec grapes cultivated in Brazil. The method was also used to investigate the changes in phenolic composition during a 10-week ripening period. Results showed an increase in anthocyanin content in grapes during the first 5-6 weeks of ripening, followed by a decrease towards week 9. The two-dimensional approach proved to be a useful tool for characterizing the complex phenolic profile of these samples.
The typical phenolic profile in grapes is characterized by its complexity both in terms of number of diverse chemical structures and their variation during ripening. Besides, the specific phenolic composition of grapes directly influences the presence of those components in the resulting wine. In this contribution, a new method based on the application of comprehensive two-dimensional liquid chromatography coupled to a diode array detector and tandem mass spectrometry has been developed to obtain the typical phenolic profile of Malbec grapes cultivated in Brazil. Moreover, the method has been demonstrated to be useful to study how the phenolic composition in grapes evolved during a 10-week ripening period. Main detected compounds in grapes and in the wine derived from them were anthocyanins, although a good number of polymeric flavan-3-ols were also tentatively identified, among other compounds. Results show how the amount of anthocyanins present in grapes was increased during ripening up to 5-6 weeks and then decreased towards week 9. The two-dimensional approach applied was demonstrated to be useful for the characterization of the complex phenolic profile of these samples, involving more than 40 different structures and has the potential to be further applied to the study of this important fraction is different grapes and wines systematically.

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