4.4 Article

FoxO3α-mediated autophagy contributes to apoptosis in cardiac microvascular endothelial cells under hypoxia

期刊

MICROVASCULAR RESEARCH
卷 104, 期 -, 页码 23-31

出版社

ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.mvr.2015.11.001

关键词

Cardiac microvascular endothelial cells; Autophagy; Apoptosis; Hypoxia; FoxO3 alpha

资金

  1. Nature Science Foundation of Shaanxi [jm4014]

向作者/读者索取更多资源

Hypoxic injury of cardiac microvascular endothelial cells (CMECs) is an important pathophysiological event in myocardial infarction, whereas, the underlying mechanism is still poorly understood. Autophagy, a highly conserved process of cellular degradation, is required for normal cardiac function and also has been implicated in various cardiovascular diseases. Here we investigated the potential role of autophagy in CMEC dysfunction under hypoxia. CMECs were isolated from SD rats. Hypoxia (6-24 h, 1% O-2) induced autophagy in CMECs as evidenced by formation of punctate LC3, increased conversion of LC3-I to LC3-II and increased p62 degradation. Importantly, hypoxia-induced apoptosis in CMECs was attenuated by 3-Methyladenine (5 mM), an autophagy inhibitor, and aggravated by rapamycin (1.0 mu g/L), an autophagy inducer. Meanwhile, hypoxia increased the nuclear localization of FoxO3 alpha, accompanying with the decreased phosphorylation of FoxO3 alpha and Akt FoxO3 alpha silencing decreased hypoxia-induced autophagy and the resultant apoptosis. Furthermore, treatment with 3-Methyladenine (10 mg/kg/day) improved the endothelial-dependent diastolic function of coronary artery in rats with myocardial infarction. These results indicated that hypoxia-induced autophagy formation in CMECs is mediated by FoxO3 alpha and contributes to hypoxic injury of hearts. 2015 Elsevier Inc. All rights reserved.

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