期刊
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
卷 27, 期 24, 页码 4056-4068出版社
WILEY
DOI: 10.1111/jcmm.17988
关键词
BMMSCs; cell-aggregates; Fas/FasL; microRNA; periodontal regeneration
This study identified the role of let-7a in the osteogenesis of BMMSCs and provided a potential method for periodontal bone regeneration. Let-7a was found to promote osteogenesis through the Fas/FasL-autophagy pathway, suggesting its promising application in periodontal bone regeneration.
Periodontal bone regeneration using bone marrow mesenchymal stem cell (BMMSC) transplantation is a promising method; however, the method for osteogenic differentiation of BMMSCs needs to be improved. In this research, we sought to identify the roles of let-7a in the osteogenesis of BMMSCs and to provide a potential method for periodontal bone regeneration. Our previous study revealed that Fas/FasL is a target of let-7a. In this study, we demonstrated that let-7a overexpression significantly enhanced BMMSC-CAs osteogenesis both in vitro and in vivo. Mechanistically, upregulation of Fas/FasL using the rfas/rfaslg plasmid obstructed the osteogenesis of BMMSCs by inhibiting autophagy. Furthermore, we confirmed that overexpression of let-7a activated autophagy and alleviated the inhibited osteogenesis by the autophagy inhibitor 3-MA and the rfas/rfaslg plasmid of BMMSCs. In general, our findings showed that let-7a promoted the osteogenesis of BMMSCs through the Fas/FasL-autophagy pathway, suggesting that the application of let-7a in BMMSC-CAs based periodontal bone regeneration could be a promising strategy.
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