期刊
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
卷 149, 期 17, 页码 15349-15364出版社
SPRINGER
DOI: 10.1007/s00432-023-05313-w
关键词
HCC; hsa-miR-21-5p; hsa-miR-155-5p; Liver cirrhosis; Monocyte subsets; In silico analysis
类别
The purpose of this study was to investigate the correlation between altered monocyte subsets distribution in liver cirrhosis (LC) and hepatocellular carcinoma (HCC) and the expression level of plasma Homo sapiens (has)-miR-21-5p and hsa-miR-155-5p. The results showed that the expression of hsa-miR-21-5p was positively correlated with intermediate monocytes, while the expression of hsa-miR-155-5p was negatively correlated with non-classical monocytes. Combining the frequency of intermediate monocytes and hsa-miR-21 could be a sensitive indicator of LC progression to HCC.
PurposeThe authors aim to investigate the altered monocytes subsets distribution in liver cirrhosis (LC) and subsequent hepatocellular carcinoma (HCC) in association with the expression level of plasma Homo sapiens (has)-miR-21-5p and hsa-miR-155-5p. A step toward non-protein coding (nc) RNA precision medicine based on the immune perturbation manifested as altered monocytes distribution, on top of LC and HCC.MethodsSeventy-nine patients diagnosed with chronic hepatitis C virus (CHCV) infection with LC were enrolled in the current study. Patients were sub-classified into LC group without HCC (n = 40), LC with HCC (n = 39), and 15 apparently healthy controls. Monocyte subsets frequencies were assessed by flow cytometry. Real-time quantitative PCR was used to measure plasma hsa-miR-21-5p and hsa-miR-155-5p expression.ResultsHsa-miR-21-5p correlated with intermediate monocytes (r = 0.30, p = 0.007), while hsa-miR-155-5p negatively correlated with non-classical monocytes (r = - 0.316, p = 0.005). ROC curve analysis revealed that combining intermediate monocytes frequency and hsa-miR-21 yielded sensitivity = 79.5%, specificity = 75%, and AUC = 0.84. In comparison, AFP yielded a lower sensitivity = 69% and 100% specificity with AUC = 0.85. Logistic regression analysis proved that up-regulation of intermediate monocytes frequency and hsa-miR-21-5p were independent risk factors for LC progression to HCC, after adjustment for co-founders.ConclusionMonocyte subsets differentiation in HCC was linked to hsa-miR-21-5p and hsa-miR-155-5p. Combined up-regulation of intermediate monocytes frequency and hsa-miR-21-5p expression could be considered a sensitive indicator of LC progression to HCC. Circulating intermediate monocytes and hsa-miR-21-5p were independent risk factors for HCC evolution, clinically and in silico proved.
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