A new mode for highly sensitive and specific detection of DNA based on exonuclease III-assisted target recycling amplification and mismatched catalytic hairpin assembly

A novel electrochemical sensing strategy was developed for highly sensitive and specific detection of DNA by combining the exonuclease III-assisted target recycling amplification with mismatched catalytic hairpin assembly. The proposed method showed high sensitivity and selectivity with a dynamic response range from 100 fM to 5 nM.