期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 24, 期 20, 页码 -出版社
MDPI
DOI: 10.3390/ijms242015084
关键词
translational readthrough; nonsense; premature termination codons oxadiazole; precision medicine; genetic diseases
Nonsense mutations cause genetic diseases, but translational readthrough-inducing drugs (TRIDs) offer a promising approach to correct these defects. The new TRIDs NV848, NV914, and NV930 have been shown to restore protein synthesis without affecting natural termination codons (NTCs).
Nonsense mutations cause several genetic diseases such as cystic fibrosis, Duchenne muscular dystrophy, beta-thalassemia, and Shwachman-Diamond syndrome. These mutations induce the formation of a premature termination codon (PTC) inside the mRNA sequence, resulting in the synthesis of truncated polypeptides. Nonsense suppression therapy mediated by translational readthrough-inducing drugs (TRIDs) is a promising approach to correct these genetic defects. TRIDs generate a ribosome miscoding of the PTC named translational readthrough and restore the synthesis of full-length and potentially functional proteins. The new oxadiazole-core TRIDs NV848, NV914, and NV930 (NV) showed translational readthrough activity in nonsense-related in vitro systems. In this work, the possible off-target effect of NV molecules on natural termination codons (NTCs) was investigated. Two different in vitro approaches were used to assess if the NV molecule treatment induces NTC readthrough: (1) a study of the translational-induced p53 molecular weight and functionality; (2) the evaluation of two housekeeping proteins' (Cys-C and beta 2M) molecular weights. Our results showed that the treatment with NV848, NV914, or NV930 did not induce any translation alterations in both experimental systems. The data suggested that NV molecules have a specific action for the PTCs and an undetectable effect on the NTCs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据