期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 24, 期 18, 页码 -出版社
MDPI
DOI: 10.3390/ijms241814190
关键词
magnetic nanoparticles; amine-functionalized magnesium ferrite; fast microwave synthesis; DNA binding
By using modified microwave-assisted synthesis, the reaction time for nucleic acid extraction can be significantly reduced, and the amine-functionalized magnesium ferrite nanoparticles show equal effectiveness in DNA extraction.
For pathogens identification, the PCR test is a widely used method, which requires the isolation of nucleic acids from different samples. This extraction can be based on the principle of magnetic separation. In our work, amine-functionalized magnesium ferrite nanoparticles were synthesized for this application by the coprecipitation of ethanolamine in ethylene glycol from Mg(II) and Fe(II) precursors. The conventional synthesis method involves a reaction time of 12 h (MgFe2O4-H & R MNP); however, in our modified method, the reaction time could be significantly reduced to only 4 min by microwave-assisted synthesis (MgFe2O4-MW MNP). A comparison was made between the amine-functionalized MgFe2O4 samples prepared by two methods in terms of the DNA-binding capacity. The experimental results showed that the two types of amine-functionalized magnesium ferrite magnetic nanoparticles (MNPs) were equally effective in terms of their DNA extraction yield. Moreover, by using a few minutes-long microwave synthesis, we obtained the same quality magnesium ferrite particles as those made through the long and energy-intensive 12-h production method. This advancement has the potential to improve and expedite pathogen identification processes, helping to better prevent the spread of epidemics.
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