期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 24, 期 13, 页码 -出版社
MDPI
DOI: 10.3390/ijms241310961
关键词
stretch-activated ion channels; ischemic cardiomyopathy; dilated cardiomyopathy
The mechanical environment of cardiac cells changes with each heartbeat and in various cardiac diseases. Cells sense and respond to mechanical cues through specialized mechano-sensors that initiate adaptive signaling cascades. By studying mechano-sensitive ion channels (MSC) in human hearts, we found differential mRNA expression of MSC genes in the atria and ventricles, as well as in different cardiac diseases. These differences may be involved in altered mechano-transduction in cardiac diseases.
The cardiac cell mechanical environment changes on a beat-by-beat basis as well as in the course of various cardiac diseases. Cells sense and respond to mechanical cues via specialized mechano-sensors initiating adaptive signaling cascades. With the aim of revealing new candidates underlying mechano-transduction relevant to cardiac diseases, we investigated mechano-sensitive ion channels (MSC) in human hearts for their chamber- and disease-preferential mRNA expression. Based on a meta-analysis of RNA sequencing studies, we compared the mRNA expression levels of MSC in human atrial and ventricular tissue samples from transplant donor hearts (no cardiac disease), and from patients in sinus rhythm (underlying diseases: heart failure, coronary artery disease, heart valve disease) or with atrial fibrillation. Our results suggest that a number of MSC genes are expressed chamber preferentially, e.g., CHRNE in the atria (compared to the ventricles), TRPV4 in the right atrium (compared to the left atrium), CACNA1B and KCNMB1 in the left atrium (compared to the right atrium), as well as KCNK2 and KCNJ2 in ventricles (compared to the atria). Furthermore, 15 MSC genes are differentially expressed in cardiac disease, out of which SCN9A (lower expressed in heart failure compared to donor tissue) and KCNQ5 (lower expressed in atrial fibrillation compared to sinus rhythm) show a more than twofold difference, indicative of possible functional relevance. Thus, we provide an overview of cardiac MSC mRNA expression in the four cardiac chambers from patients with different cardiac diseases. We suggest that the observed differences in MSC mRNA expression may identify candidates involved in altered mechano-transduction in the respective diseases.
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