期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 24, 期 20, 页码 -出版社
MDPI
DOI: 10.3390/ijms242015489
关键词
fatty liver; nonalcoholic steatohepatitis; chemokine; neutrophil; interleukin-8
This study aimed to investigate the role of neutrophil infiltration in the transition from fatty liver to nonalcoholic steatohepatitis (NASH) by examining hepatic overexpression of interleukin-8 (IL8). The results showed that IL8 overexpression in mice fed a high-fat diet led to neutrophil infiltration, exacerbated liver injury and inflammation, and promoted fibrosis.
Nonalcoholic steatohepatitis (NASH) is an advanced stage of fatty liver disease characterized by liver damage, inflammation, and fibrosis. Although neutrophil infiltration is consistently observed in the livers of patients with NASH, the precise role of neutrophil-recruiting chemokines and infiltrating neutrophils in NASH pathogenesis remains poorly understood. Here, we aimed to elucidate the role of neutrophil infiltration in the transition from fatty liver to NASH by examining hepatic overexpression of interleukin-8 (IL8), a major chemokine responsible for neutrophil recruitment in humans. Mice fed a high-fat diet (HFD) for 3 months developed fatty liver without concurrent liver damage, inflammation, and fibrosis. Subsequent infection with an adenovirus overexpressing human IL8 for an additional 2 weeks increased IL8 levels, neutrophil infiltration, and liver injury in mice. Mechanistically, IL8-induced liver injury was associated with the upregulation of components of the NADPH oxidase 2 complex, which participate in neutrophil oxidative burst. IL8-driven neutrophil infiltration promoted macrophage aggregate formation and upregulated the expression of chemokines and inflammatory cytokines. Notably, IL8 overexpression amplified factors associated with fibrosis, including collagen deposition and hepatic stellate cell activation, in HFD-fed mice. Collectively, hepatic overexpression of human IL8 promotes neutrophil infiltration and fatty liver progression to NASH in HFD-fed mice.
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