期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 24, 期 17, 页码 -出版社
MDPI
DOI: 10.3390/ijms241713345
关键词
NRF2; NQO1; KEAP1; lipodystrophy; lipogenesis; hydrodynamic tail vein injection; liver
This study investigated the possibility of activating NRF2 signaling or directly elevating NQO1 levels in the liver to reduce steatosis associated with lipodystrophy. Through hydrodynamic tail vein injection of pCAG-Nrf2 and pCAG-Nqo1 expression vectors in malnourished mice, it was found that both methods protected the liver from damage, with the pCAG-Nqo1 vector being more effective.
Lipodystrophy is a disorder featuring loss of normal adipose tissue depots due to impaired production of normal adipocytes. It leads to a gain of fat deposition in ectopic tissues such as liver and skeletal muscle that results in steatosis, dyslipidemia, and insulin resistance. Previously, we established a Rosa(NIC/NIC)::AdiCre lipodystrophy model mouse. The lipodystrophic phenotype that included hepatomegaly accompanied with hepatic damage due to higher lipid accumulation was attenuated substantially by amplified systemic NRF2 signaling in mice with hypomorphic expression of Keap1; whole-body Nrf2 deletion abrogated this protection. To determine whether hepatic-specific NRF2 signaling would be sufficient for protection against hepatomegaly and fatty liver development, direct, powerful, transient expression of Nrf2 or its target gene Nqo1 was achieved by administration through hydrodynamic tail vein injection of pCAG expression vectors of dominant-active Nrf2 and Nqo1 in Rosa(NIC/NIC)::AdiCre mice fed a 9% fat diet. Both vectors enabled protection from hepatic damage, with the pCAG-Nqo1 vector being the more effective as seen with a similar to 50% decrease in hepatic triglyceride levels. Therefore, activating NRF2 signaling or direct elevation of NQO1 in the liver provides new possibilities to partially reduce steatosis that accompanies lipodystrophy.
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