期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 24, 期 18, 页码 -出版社
MDPI
DOI: 10.3390/ijms241814125
关键词
cystathionine gamma-synthase; Glycine max; methionine biosynthesis; soybean protein; sulfur-containing amino acid; methionine
Genetically modifying soybeans to increase the content of the sulfur-containing amino acid methionine can improve the nutritional quality of soybean protein.
Soybean (Glycine max (L.) Merr.) is an important source of plant protein, the nutritional quality of which is considerably affected by the content of the sulfur-containing amino acid, methionine (Met). To improve the quality of soybean protein and increase the Met content in seeds, soybean cystathionine gamma-synthase 2 (GmCGS2), the first unique enzyme in Met biosynthesis, was overexpressed in the soybean cultivar Jack, producing three transgenic lines (OE3, OE4, and OE10). We detected a considerable increase in the content of free Met and other free amino acids in the developing seeds of the three transgenic lines at the 15th and 75th days after flowering (15D and 75D). In addition, transcriptome analysis showed that the expression of genes related to Met biosynthesis from the aspartate-family pathway and S-methyl Met cycle was promoted in developing green seeds of OE10. Ultimately, the accumulation of total amino acids and soluble proteins in transgenic mature seeds was promoted. Altogether, these results indicated that GmCGS2 plays an important role in Met biosynthesis, by providing a basis for improving the nutritional quality of soybean seeds.
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