LRG1 Promotes ECM Integrity by Activating the TGF-beta Signaling Pathway in Fibroblasts

Leucine-rich alpha-2-glycoprotein 1 (LRG1) mediates skin repair and fibrosis by stimulating the transforming growth factor-beta (TGF-beta) signaling pathway. In the present study, we investigated the effect of LRG1 on extracellular matrix (ECM) integrity in fibroblasts, as well as on skin aging. The treatment of dermal fibroblasts with purified recombinant human LRG1 increased type I collagen secretion and decreased matrix metalloproteinase-1 secretion. Additionally, LRG1 promoted SMAD2/SMAD3 phosphorylation in a pattern similar to that of TGF- beta 1 treatment. An inhibitor of TGF- beta receptor 1 abolished LRG1-induced SMAD2 phosphorylation. RNA sequencing identified extracellular region, extracellular space, and extracellular matrix as the main Gene Ontology terms in the differentially expressed genes of fibroblasts treated with or without LRG1. LRG1 increased TGF-beta 1 mRNA levels, suggesting that LRG1 partially transactivates the expression of TGF-beta 1. Furthermore, an increased expression of type I collagen was also observed in fibroblasts grown in three-dimensional cultures on a collagen gel mimicking the dermis. LRG1 mRNA and protein levels were significantly reduced in elderly human skin tissues with weakened ECM integrity compared to in young human skin tissues. Taken together, our results suggest that LRG1 could retard skin aging by activating the TGF- beta signaling pathway, increasing ECM deposition while decreasing its degradation.

Automated summary

LRG1 plays a role in skin repair and fibrosis by stimulating the TGF-β signaling pathway. It increases type I collagen secretion and decreases matrix metalloproteinase-1 secretion in fibroblasts. LRG1 can activate the TGF-β pathway and promote collagen deposition while reducing its degradation, potentially slowing down skin aging.