期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 24, 期 14, 页码 -出版社
MDPI
DOI: 10.3390/ijms241411815
关键词
human corneal epithelium; transient receptor potential channel vanilloid 1; L-carnitine; intracellular Ca2+ signaling; planar patch-clamp technique; cell volume; hypertonic cell shrinkage
Tear film hyperosmolarity induces dry eye syndrome through TRPV1 activation. L-carnitine, a therapeutic agent, protects against hypertonicity-induced response. This study shows that L-carnitine inhibits TRPV1 activation by blocking cell volume shrinkage induced by hypertonicity.
Tear film hyperosmolarity induces dry eye syndrome (DES) through transient receptor potential vanilloid type 1 (TRPV1) activation. L-carnitine is a viable therapeutic agent since it protects against this hypertonicity-induced response. Here, we investigated whether L-carnitine inhibits TRPV1 activation by blocking heat- or capsaicin-induced increases in Ca2+ influx or hyperosmotic stress-induced cell volume shrinkage in a human corneal epithelial cell line (HCE-T). Single-cell fluorescence imaging of calcein/AM-loaded cells or fura-2/AM-labeled cells was used to evaluate cell volume changes and intracellular calcium levels, respectively. Planar patch-clamp technique was used to measure whole-cell currents. TRPV1 activation via either capsaicin (20 & mu;mol/L), hyperosmolarity (& AP;450 mosmol/L) or an increase in ambient bath temperature to 43 & DEG;C induced intracellular calcium transients and augmented whole-cell currents, whereas hypertonicity induced cell volume shrinkage. In contrast, either capsazepine (10 & mu;mol/L) or L-carnitine (1-3 mmol/L) reduced all these responses. Taken together, L-carnitine and capsazepine suppress hypertonicity-induced TRPV1 activation by blocking cell volume shrinkage.
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